MIP1 / YOR330C Overview
- Standard Name
- MIP1 1
- Systematic Name
- YOR330C
- SGD ID
- SGD:S000005857
- Feature Type
- ORF , Verified
- Description
- Mitochondrial DNA polymerase gamma; single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoan complex of catalytic and accessory subunits; polymorphic in yeast, petites occur more frequently in some lab strains; human ortholog POLG complements yeast mip1 mutant; mutations in human POLG associated with Alpers-Huttenlocher syndrome (AHS), progressive external ophthalmoplegia (PEO), parkinsonism, other mitochondrial diseases 2 3 4 5 6 7 8 9
- Name Description
- MItochondrial DNA Polymerase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mip1p is 1254 amino acids long, extremely long-lived, and low in abundance; contains 9 mitochondrial DNA-directed DNA-polymerase family A domains
- Length (a.a.)
- 1254
- Mol. Weight (Da)
- 143532.1
- Isoelectric Point
- 9.03
- Median Abundance (molecules/cell)
- 562 +/- 465
- Half-life (hr)
- >= 100
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MIP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Mitochondrial DNA polymerase and 3'-5' exonuclease involved in mitochondrial DNA replication and mitochondrial DNA catabolism during nitrogen starvation
View computational annotations
Molecular Function
- Manually Curated
- enables 3'-5' exonuclease activity (IDA)
- enables DNA-directed DNA polymerase activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to nitrogen starvation (IMP)
- involved in mitochondrial DNA catabolic process (IMP)
- involved in mitochondrial DNA replication (IDA, IMP)
- involved in mitochondrial genome maintenance (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (IDA, HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants grow slowly, have abnormal vacuolar morphology, accumulate glycogen, cannot respire or use various nitrogen sources, are sensitive to oxidative stress and desiccation, and have shortened lifespan and small cell size; missense mutants that correspond to human Alpers disease mutations show increased mitochondrial DNA point mutations; heterozygous diploid nulls are haploinsufficient
Classical Genetics
- dominant negative
- null
- overexpression
- reduction of function
- heat sensitivity: increased
- mitochondrial genome maintenance: absent
- mitochondrial genome maintenance: decreased
- mutation frequency: increased
- petite
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory metabolism: absent
- respiratory metabolism: decreased
- respiratory metabolism: normal
- vegetative growth: decreased
- conditional
- gain of function
- null
- acid pH resistance: decreased
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: decreased
- mitochondrial genome maintenance: absent
- mitochondrial genome maintenance: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: increased
- toxin resistance: decreased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: abnormal
- vegetative growth: decreased rate
- viable
- unspecified
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MIP1 is homologous to human POLG, and has been used to study chronic progressive external ophthalmoplegia, Alpers syndrome, progeria, and peripheral nervous system disease
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mip1p interacts physically with proteins involved in chromatin organization; MIP1 interacts genetically with genes involved in lipid metabolism
202 total interactions for 166 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 1
- Cross-Linking-MS (XL-MS): 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 118
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 6
- Positive Genetic: 47
- Synthetic Growth Defect: 3
- Synthetic Lethality: 2
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-05-22
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.