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SNF8 / YPL002C Overview

Standard Name
SNF8 1
Systematic Name
YPL002C
SGD ID
SGD:S000005923
Aliases
VPS22 16 17 , VPL14 17
Feature Type
ORF , Verified
Description
Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression 2
Name Description
Sucrose NonFermenting 1 3
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Analyze Sequence

S288C only

BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation

S288C vs. other species

BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi

S288C vs. other strains

Strain Alignment | Variant Viewer

Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
233
Mol. Weight (Da)
26949.3
Isoelectric Point
5.43
Median Abundance (molecules/cell)
944 +/- 575
Half-life (hr)
8.1

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all SNF8 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Summary
Subunit of the ESCRT II complex, which is required for the downregulation of cell-surface receptors and for the final membrane scission step during endocytosis; involved in carbon catabolite repression of Pol II transcription by glucose, protein targeting to the vacuole, ATP export, and ubiquitin-dependent protein catabolism via the multivesicular body sorting pathway

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Summary
SNF8/YPL002C is a non-essential gene; null mutants are viable but exhibit a range of phenotypes including decreased resistance to acidic pH, decreased biofilm formation, abnormal colony appearance, decreased desiccation resistance, decreased endocytosis, increased heat sensitivity, decreased resistance to hyperosmotic stress, decreased innate thermotolerance, absent invasive growth, decreased oxidative stress resistance, decreased peroxisomal transport, decreased replicative lifespan, decreased RNA accumulation, decreased sporulation efficiency, increased stress resistance, decreased telomere length, decreased toxin resistance, decreased transposable element transposition, absent utilization of carbon sources, decreased rate of nitrogen source utilization, abnormal vacuolar morphology, and decreased vegetative growth rate. Reduction of function mutants show abnormal vacuolar transport, while overexpression of SNF8 results in decreased vegetative growth rate.

Classical Genetics

null
reduction of function

Large-scale Survey

null
overexpression
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Summary
Snf8p interacts physically with proteins involved in transport; SNF8 interacts genetically with genes involved in transcription

176 total interactions for 128 unique genes

Physical Interactions

  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 4
  • Co-crystal Structure: 3
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 19

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 52
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 4
  • Positive Genetic: 10
  • Synthetic Growth Defect: 25
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 24
  • Synthetic Rescue: 6
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Summary
SNF8 transcription is regulated by Sfp1p during response to stress
Regulators
1
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-02-07

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.

Primary
51
Additional
45
Reviews
33

Resources

AlphaFold Protein Structure | Entrez Gene | Entrez RefSeq Protein | FungiDB | Search all NCBI | UniParc | UniProtKB