CAM1 / YPL048W Overview
- Standard Name
- CAM1 1
- Systematic Name
- YPL048W
- SGD ID
- SGD:S000005969
- Aliases
- TEF3 3 , CPBP 4
- Feature Type
- ORF , Verified
- Description
- One of two isoforms of the gamma subunit of eEF1B; stimulates the release of GDP from eEF1A (Tef1p/Tef2p) post association with the ribosomal complex with eEF1Balpha subunit; nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids 1 2 3 4
- Name Description
- Calcium And Membrane-binding protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cam1p is 415 amino acids long, of moderate half-life; contains glutathione domains; protein abundance decreases under nitrogen starvation or treatment with DNA-damaging agents or rapamycin
- Length (a.a.)
- 415
- Mol. Weight (Da)
- 47091.1
- Isoelectric Point
- 8.74
- Median Abundance (molecules/cell)
- 23200 +/- 5549
- Half-life (hr)
- 10.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CAM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Promoter-binding guanyl-nucleotide exchange factor that binds calcium and phospholipids, and affects transcription, ribosome biogenesis, and translational elongation and termination
View computational annotations
Molecular Function
- Manually Curated
- enables calcium ion binding (IDA)
- enables core promoter sequence-specific DNA binding (IDA)
- enables DNA-binding transcription factor activity (IMP)
- enables guanyl-nucleotide exchange factor activity (IDA)
- enables phospholipid binding (IDA)
Biological Process
- Manually Curated
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of translational termination (IGI)
- involved in translational elongation (IDA)
Cellular Component
- Manually Curated
- located in cytoplasmic stress granule (HDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants show decreased fitness and hyperosmotic stress resistance, but increased resistance to oxidative stress and to the antibiotic paromomycin
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- CAM1 interacts genetically with loci involved in transcription and RNA metabolism; Cam1p interacts physically with proteins involved in regulation of translation and organelle organization
219 total interactions for 192 unique genes
Physical Interactions
- Affinity Capture-MS: 53
- Affinity Capture-RNA: 5
- Biochemical Activity: 2
- Co-purification: 1
- PCA: 2
- Proximity Label-MS: 2
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 3
- Negative Genetic: 121
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Positive Genetic: 24
- Synthetic Lethality: 1
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CAM1 encodes a calcium phospholipid-binding transcriptional coactivator homologous to elongation factor-1 gamma (EF-1 gamma). Cam1p regulates expression of the MXR1 gene encoding methionine sulfoxide reductase A, which maintains the function of many proteins by reversing oxidation of methionine residues. Cam1p may act as a response regulator during environmental stress conditions.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-06-24
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.