Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
41 entries for 21 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| apoptosis: increased | classical genetics | reduction of function Allele: rad53-21 | W303 | Media: nutrient limitation Details: more rapid age-related apoptotic DNA degradation | Weinberger M, et al. (2007) PMID:17710147 | |
| bud morphology: abnormal | classical genetics | null Allele: rad53-Δ | S288C | Details: enhanced Swe1p- and Ecm25p-dependent formation of elongated buds | Duan X, et al. (2021) PMID:34010635 | |
| bud morphology: abnormal | classical genetics | null Allele: rad53-Δ | S288C | 100 mM hydroxyurea | Details: further enhancement of Swe1p- and Ecm25p-dependent bud elongation that occurs in response to stress, such as after treatment with a sub-lethal dosage of HU | Duan X, et al. (2021) PMID:34010635 |
| budding index: increased | classical genetics | reduction of function Allele: rad53-21 | W303 | Media: nutrient limitation | Weinberger M, et al. (2007) PMID:17710147 | |
| cell cycle progression in G1 phase: increased | classical genetics | reduction of function Allele: rad53-21 | W303 | Media: nutrient limitation Details: decreased efficiently of arrest in G1 phase | Weinberger M, et al. (2007) PMID:17710147 | |
| cell cycle progression in S phase: increased duration | classical genetics | reduction of function Allele: rad53-1 | Other | Media: glycerol medium Details: 22% of cells have 2N DNA content, compared to 46% of wild type cells | Lebedeva MA and Shadel GS (2007) PMID:17721079 | |
| chemical compound accumulation: decreased | classical genetics | null Allele: rad53-Δ | Other | reactive oxygen species | Treatment: double-strand break (DSB) Details: levels of ROS in the null mutant with one DSB are down-regulated compared to WT strains with one DSB as determined by confocal microscopy and flow cytometry | Li X, et al. (2024) PMID:39018963 |
| chronological lifespan: decreased | classical genetics | reduction of function Allele: rad53-21 | W303 | Media: high glucose, 10% glucose (intial concentration in media) | Weinberger M, et al. (2010) PMID:21076178 | |
| competitive fitness: decreased | competitive growth | overexpression | S288C | Details: decreased abundance within the barFLEX overexpression collection arrayed after 20 generations of pooled growth | Douglas AC, et al. (2012) PMID:23050238 | |
| haploinsufficient | heterozygous diploid, competitive growth genome-wide fitness profiling | null Allele: rad53-Δ | S288C | Media: turbidostat growth in FPM medium Details: Relative growth score: -0.0052 | Pir P, et al. (2012) PMID:22244311 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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