AFT2 / YPL202C Overview
- Standard Name
- AFT2 1
- Systematic Name
- YPL202C
- SGD ID
- SGD:S000006123
- Feature Type
- ORF , Verified
- Description
- Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication 1 2 3 4
- Name Description
- Activator of Fe (iron) Transcription 1
- Paralog
- AFT1 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- AFT2 has a paralog, AFT1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 416
- Mol. Weight (Da)
- 47113.4
- Isoelectric Point
- 9.1
- Median Abundance (molecules/cell)
- 139 +/- 119
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all AFT2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription factor that regulates cellular iron homeostasis and oxidative stress response; localizes to both nucleus and mitochondria
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cardiolipin metabolic process (IMP)
- involved in cellular response to iron ion starvation (IGI)
- involved in intracellular iron ion homeostasis (IGI)
- involved in positive regulation of transcription by RNA polymerase II (IDA, IMP, IGI)
- involved in regulation of phosphate transmembrane transport (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to heat, desiccation, and various chemicals; heterozygous diploid null is haploinsufficient
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The aft2 null mutant is viable; the null mutant of paralog aft1 is viable; the aft2 aft1 double mutant is inviable or displays a growth defect.
84 total interactions for 65 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 4
- Co-purification: 1
- Reconstituted Complex: 6
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 49
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 1
- Positive Genetic: 3
- Synthetic Growth Defect: 10
- Synthetic Lethality: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- AFT1 and AFT2 encode paralogous low-iron-sensing transcriptional activators that regulate the expression of iron uptake and storage genes. Aft1p and Aft2p activate gene expression under iron-deficient conditions by binding to the same promoter elements, containing 5'-RCACCC-3' motifs. Aft1p is considered the primary regulator of iron homeostasis, but Aft2 can partially compensate for the loss of Aft1. The activities of Aft1p and Aft2p are regulated by monothiol glutaredoxins Grx3p and Grx4p, and exportin Msn5p. Aft1p cycles between the nucleus and cytosol, favoring cytosolic localization under iron-replete conditions, which induce multimerization of Aft1p/2p and favor export from the nucleus via interaction with the exportin Msn5p. Under low-iron conditions, Aft1p and Aft2p accumulates in the nucleus where it binds to and activates genes involved in iron uptake, transport, and storage, known collectively as the iron regulon.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.