FAS2 / YPL231W Overview


Standard Name
FAS2 1
Systematic Name
YPL231W
SGD ID
SGD:S000006152
Feature Type
ORF , Verified
Description
Alpha subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities 2 3 4
Name Description
Fatty Acid Synthetase
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
FAS2 is located on the left arm of chromosome XVI between SSO1 plasma membrane t-SNARE and USV1 zinc finger protein; coding sequence is 5664 nucleotides long with 51 SNPs, 8 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Fas2p is 1887 amino acids long, longer-lived, high in abundance; contains acyl carrier domain, type 1 helical domain, 4'-phosphopantetheinyl transferase domain, and two beta-ketoacyl synthase domains; acetylated on 43 lysines, succinylated on 17 lysines, sumoylated on K1826, phosphorylated on 93 residues
Length (a.a.)
1887
Mol. Weight (Da)
206899.6
Isoelectric Point
5.11
Median Abundance (molecules/cell)
52956 +/- 28255
Half-life (hr)
11.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all FAS2 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Subunit of cytoplasmic fatty acid synthase, contributes acyl-carrier, beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities; involved in long-chain fatty acid biosynthesis

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; null mutant is rescuable by fatty acid supplement; conditional mutations cause increased heat sensitivity; overexpression causes slow growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Fas2p interacts physically with proteins involved in translation; FAS2 interacts genetically with genes involved in transcription

471 total interactions for 429 unique genes

Physical Interactions

  • Affinity Capture-MS: 106
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 5
  • Co-crystal Structure: 3
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 2
  • Protein-RNA: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 2

Genetic Interactions

  • Negative Genetic: 296
  • Phenotypic Enhancement: 2
  • Positive Genetic: 39
  • Synthetic Rescue: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
FAS2 promoter is bound by Cin5p, Med4p, and Reb1p in response to heat; FAS2 transcription is regulated by Sfp1p in response to stress; FAS2 transcription is upregulated by Ino4p during phospholipid biosynthesis
Regulators
13
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2010-04-24

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
82
Additional
115
Reviews
60

Resources