Phenotype Help

GLN1 / YPR035W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Essential gene in S288C; reduction of function leads to hypoosmotic stress sensitivity rescuable by osmotic support in growth medium; non-essential gene in Sigma1278b, where null mutation causes glutamine auxotrophy and reduced filamentous growth

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

10 entries for 9 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
auxotrophy
classical geneticsunspecifiedSigma1278b glutamineMitchell AP (1985) PMID:2865193
competitive fitness: increased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 1.002
Breslow DK, et al. (2008) PMID:18622397
filamentous growth: decreased
systematic mutation setnull
Allele: gln1-Δ
Sigma1278bDetails: greatly decreased
Jin R, et al. (2008) PMID:17989363
haploinsufficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: gln1-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: -0.0051
Pir P, et al. (2012) PMID:22244311
haploinsufficient
heterozygous diploid, systematic mutation set null
Allele: gln1-Δ
S288COhnuki S and Ohya Y (2018) PMID:29768403
hypoosmotic stress resistance: decreased
classical geneticsreduction of function
Allele: gln1-(hpo1-1)
S288C1 M glucitolDetails: mutant requires osmotic support from sorbitol
Shimizu J, et al. (1997) PMID:12501331
invasive growth: absent
systematic mutation setnull
Allele: gln1-Δ
Sigma1278bJin R, et al. (2008) PMID:17989363
inviable
systematic mutation setnull
Allele: gln1-Δ
S288CGiaever G, et al. (2002) PMID:12140549
killer toxin resistance: decreased
systematic mutation setconditionalS288CTemperature: elevated temperature, 34 °C
Details: K28 killer toxin
Carroll SY, et al. (2009) PMID:19853568
starvation resistance: decreased
heterozygous diploid, competitive growth

pooled mutant strains were subjected to low nutrient stress, then dead cells were separated and their mutations identified

null
Allele: gln1-Δ
S288CDavey HM, et al. (2012) PMID:22356628
Showing 1 to 10 of 10 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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