SEC8 / YPR055W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

SEC8/YPR055W is an essential gene in reference strain S288C; null deletion is inviable, but partial loss or conditional alleles reveal broad trafficking and organelle defects: reduced protein secretion and increased protein accumulation at high temperature, abnormal protein distribution, endomembrane morphology defects, and altered ER and Golgi distribution; nucleolar size is reduced in sec8-6 and sec8-9, and mitochondrial morphology is abnormal upon repression (doxycycline). Mutants show decreased toxin resistance to Aβ42 at permissive temperature and altered drug sensitivities that depend on allele: sec8-6 is hypersensitive to camptothecin, micafungin, and acivicin, whereas sec8-9 is hypersensitive to micafungin but more resistant to camptothecin and acivicin. Heterozygous deletions are haploinsufficient and display decreased resistance to fenpropimorph, latrunculin A, and tubulozole. Vegetative growth is reduced under misexpression, and sec8-9 causes absent sporulation at 34°C.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources