ROX1 / YPR065W Overview
- Standard Name
- ROX1 1
- Systematic Name
- YPR065W
- SGD ID
- SGD:S000006269
- Aliases
- REO1 8 9
- Feature Type
- ORF , Verified
- Description
- Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance 2 3 4 5 6 7
- Name Description
- Regulation by OXygen 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 368
- Mol. Weight (Da)
- 41856.7
- Isoelectric Point
- 10.46
- Median Abundance (molecules/cell)
- 1157 +/- 1025
- Half-life (min)
- 29.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ROX1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription factor that represses expression of hypoxia-induced genes in the presence of oxygen; also represses target genes during osmotic stress
View computational annotations
Molecular Function
- Manually Curated
- enables cis-regulatory region sequence-specific DNA binding (IDA)
- enables DNA binding, bending (IDA)
- enables DNA-binding transcription repressor activity, RNA polymerase II-specific (IMP)
- enables sequence-specific DNA binding (IDA)
- enables transcription corepressor activity (IMP)
Biological Process
- Manually Curated
- involved in cellular response to increased oxygen levels (IMP)
- involved in cellular response to osmotic stress (IMP, IGI)
- involved in cellular response to salt stress (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- anaerobic growth: decreased
- cell cycle progression in G1 phase: delayed
- competitive fitness: decreased
- competitive fitness: increased
- killer toxin resistance: increased
- metal resistance: decreased
- oxidative stress resistance: increased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- stress resistance: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
273 total interactions for 218 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 8
- PCA: 1
- Reconstituted Complex: 6
Genetic Interactions
- Dosage Lethality: 4
- Negative Genetic: 201
- Phenotypic Enhancement: 6
- Phenotypic Suppression: 8
- Positive Genetic: 28
- Synthetic Growth Defect: 3
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ROX1 encodes an alpha helix transcription factor of the high mobility group (HMG) family. Rox1p binds 5'-YYHATTGTTCTC-3' motifs in the promoters of diverse hypoxic genes and prevents their expression in aerobic conditions. Mot3p enhances this aerobic repression by helping Rox1p recruit the Ssn6p/Tup1p general co-repressor complex and by stabilizing the Rox1p-Ssn6p interaction. Rox1p is important for growth and modulation of ergosterol levels in response to high osmolarity. Rox1p binds telomeric X elements and controls the propagation of telomeric silencing toward centromeres in response to stress and metabolic changes. ROX1 transcription is regulated by Hap1p. When oxygen levels drop, Hap1p is inactivated and ROX1 is not expressed, allowing aerobic expression of genes involved in the hypoxic response.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.