SPN1 / YPR133C Overview

Standard Name
SPN1 1
Systematic Name
IWS1 2
Feature Type
ORF , Verified
Protein involved in RNA polymerase II transcription; also required for histone modifications and splicing; constitutively recruited to the CYC1 promoter and required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain is highly conserved throughout eukaryotes; mutations confer an Spt- phenotype 1 2 3 4 5 6
Name Description
Suppresses Postrecruitment functions gene Number 1 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
8336 +/- 4165
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all SPN1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Non-DNA binding transcription factor; involved in regulation of transcription from RNA Polymerase II and poly(A)+ mRNA export from nucleus

View computational annotations

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

679 total interactions for 475 unique genes

Physical Interactions

  • Affinity Capture-MS: 73
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 16
  • Co-crystal Structure: 1
  • PCA: 4
  • Proximity Label-MS: 1
  • Reconstituted Complex: 7

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 439
  • Phenotypic Suppression: 1
  • Positive Genetic: 83
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 5
  • Synthetic Rescue: 20
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

SPN1 encodes a highly-conserved chromatin-associated transcription factor with a conserved central domain composed of eight alpha-helices similar in structure to TFIIS. Spn1p occupies poised promoters in the uninduced state, along with RNA polymerase II. Histone chaperone Spt6p and the Swi/Snf complex are recruited upon activation. Spt6p promotes reassembly of nucleosomes following passage of RNA polymerase II. By interacting with both RNA polymerase II and Spt6p, Spn1p links the regulation of the poised promoter to chromatin architecture, and is an important regulator of the Spt6-nucleosome interaction. Binding of Spn1p at poised promoters blocks the recruitment of the Swi/Snf complex in the uninduced state and serves as a platform for the recruitment of Spt6p during the activated state. Spn1p and Spt6p have also been implicated in mRNA processing.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.