TDH3 / YGR192C Overview
- Standard Name
- TDH3 1
- Systematic Name
- YGR192C
- SGD ID
- SGD:S000003424
- Aliases
- GLD1 , HSP35 , HSP36 , SSS2 10 , GAPDH 8 , GPD 11
- Feature Type
- ORF , Verified
- Description
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; moonlighting role as an AU-rich mRNA binding protein that binds to the FAS1 mRNA and controls periodic abundance of Fas1p 1 2 3 4 5
- Name Description
- Triose-phosphate DeHydrogenase 1
- Paralog
- TDH2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Somewhat long-lived protein with Rossmann-fold and glyceraldehyde-3-phosphate dehydrogenase domains; shares several domains with Tdh1p and Tdh2p; undergoes various post-translational modifications at 64 residues, including N-glycosylation, phosphorylation, ubiquitinylation, methylation, acetylation, and succinylation
- Length (a.a.)
- 332
- Mol. Weight (Da)
- 35745.0
- Isoelectric Point
- 6.96
- Median Abundance (molecules/cell)
- 746358 +/- 666753
- Half-life (hr)
- 12.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TDH3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Glyceraldehyde-3-phosphate dehydrogenase involved in glycolysis, gluconeogenesis, apoptosis, and the metabolism of reactive oxygen species; binds RNA; localizes to cytoplasm, mitochondria, lipid particles, and the cell wall
View computational annotations
Molecular Function
- Manually Curated
- enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity (IDA)
- enables heme binding (IMP)
- enables melatonin binding (IDA)
- enables mRNA binding (IDA)
- enables RNA binding (IDA)
Biological Process
- Manually Curated
- involved in apoptotic process (IMP)
- involved in gluconeogenesis (IEP)
- involved in glycolytic process (IEP)
- involved in heme transport (IMP)
- involved in reactive oxygen species metabolic process (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- is active in cytosol (IDA, HDA)
- located in fungal-type cell wall (IDA)
- located in lipid droplet (IDA)
- located in mitochondrion (HDA, IDA)
- located in nucleus (HDA)
- located in plasma membrane (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is temperature sensitive, has a decreased growth rate and reduced competitive fitness in various growth media; null mutant displays increased survival following a ramped heat stimulus; null mutant has decreased desiccation resistance, and acid pH resistance but increased resistance to zinc deficiency; haploinsufficiency results in chromosome instability; overexpression results in a decreased growth rate, and an extended G1 phase
Classical Genetics
- null
- acid pH resistance: decreased
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: decreased
- colony sectoring: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: decreased
- heat sensitivity: increased
- metal resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: increased
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased rate
- viable
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The tdh3 null mutant is viable; the null mutant of paralog tdh2 is viable; the tdh3 tdh2 double mutant is inviable or displays a growth defect; the tdh1 tdh2 tdh3 triple mutant is inviable; Tdh3p interacts physically with proteins involved in translation, TDH3 interacts genetically with genes involved in transcription and cell cycle
427 total interactions for 340 unique genes
Physical Interactions
- Affinity Capture-MS: 198
- Affinity Capture-RNA: 19
- Affinity Capture-Western: 8
- Co-fractionation: 1
- Co-purification: 2
- PCA: 4
- Proximity Label-MS: 7
- Reconstituted Complex: 1
- Two-hybrid: 9
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 3
- Negative Genetic: 140
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 2
- Positive Genetic: 16
- Synthetic Growth Defect: 4
- Synthetic Lethality: 7
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Transcription of TDH3 is positively regulated by Gcr2p, and is also regulated by Gcr1p, Tap1p, and Tye7p
- Regulators
- 25
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-12-12
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.