TOR1 / YJR066W Overview
- Standard Name
- TOR1 1
- Systematic Name
- YJR066W
- SGD ID
- SGD:S000003827
- Aliases
- DRR1 42
- Feature Type
- ORF , Verified
- Description
- PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; indole-3-acetic acid, an ATP-competitive TORC1 inhibitor, accumulates upon entry into stationary phase; malonyl-CoA is a direct ATP-competitive inhibitor of TORC1; TOR1 has a paralog, TOR2, that arose from the whole genome duplication 2 3 4 5 6 7 8 9 10 11 12 13 14
- Name Description
- Target Of Rapamycin 1
- Paralog
- TOR2 12
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TOR1 has a paralog, TOR2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 2470
- Mol. Weight (Da)
- 281134.4
- Isoelectric Point
- 7.23
- Median Abundance (molecules/cell)
- 1323 +/- 878
- Half-life (hr)
- 7.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TOR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein kinase subunit of TORC1; involved in signal transduction, regulation of cell growth and autophagy, and the cellular response to DNA metabolism; localizes to cellular membranes, nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (IMP)
- enables protein serine/threonine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in cellular response to heat (IGI)
- involved in cellular response to nitrogen starvation (IGI)
- involved in cellular response to oxidative stress (IGI)
- involved in DNA damage response (IMP)
- involved in fungal-type cell wall organization (IMP)
- involved in meiotic cell cycle (IMP)
- involved in mitochondria-nucleus signaling pathway (IMP)
- involved in negative regulation of autophagy (IGI)
- involved in nucleolar large rRNA transcription by RNA polymerase I (IMP)
- involved in peptidyl-serine phosphorylation (IDA)
- involved in regulation of cell cycle (IMP)
- involved in regulation of snRNA pseudouridine synthesis (IGI)
- involved in regulation of sphingolipid biosynthetic process (IMP)
- involved in response to endoplasmic reticulum stress (IMP)
- involved in ribosome biogenesis (IMP)
- involved in TOR signaling (IMP)
- involved in translational initiation (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in endosome membrane (IDA)
- located in fungal-type vacuole membrane (IDA, HDA)
- located in Golgi membrane (IDA)
- located in nucleus (IDA)
- located in plasma membrane (IDA)
- part of TORC1 complex (IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows increased chronological and replicative lifespan, increased thermotolerance and oxidative stress resistance, higher accumulation of glycogen and increased entry into stationary phase; null mutation causes increased sensitivity to rapamycin; overexpression leads to rapamycin resistance; in systematic studies null mutant is sensitive to caffeine, camptothecin and ethanol, but resistant to acetate, bleomycin, fenpropimorph
Classical Genetics
- null
- alkaline pH resistance: increased
- apoptosis: decreased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased rate
- chronological lifespan: increased
- cold sensitivity: decreased
- entry into G0 (stationary phase): increased
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- hyperosmotic stress resistance: increased
- innate thermotolerance: decreased
- innate thermotolerance: increased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- mitotic recombination: increased rate
- mutation frequency: decreased
- oxidative stress resistance: increased
- protein/peptide accumulation: increased
- protein/peptide modification: decreased
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory metabolism: increased
- RNA accumulation: decreased
- RNA accumulation: increased
- vegetative growth: decreased
- viability: increased
- unspecified
- activation
- overexpression
- null
- auxotrophy
- chemical compound accumulation: increased
- chronological lifespan: increased
- competitive fitness: decreased
- haploinsufficient
- heat sensitivity: increased
- metal resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- stress resistance: increased
- vacuolar morphology: abnormal
- viable
- overexpression
- unspecified
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The tor1 null mutant is viable; the null mutant of paralog tor2 is inviable; the tor1 tor2 double mutant displays negative genetic interactions.
842 total interactions for 501 unique genes
Physical Interactions
- Affinity Capture-MS: 52
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 57
- Biochemical Activity: 21
- Co-fractionation: 50
- Co-localization: 2
- Co-purification: 6
- PCA: 37
- Reconstituted Complex: 6
- Two-hybrid: 14
Genetic Interactions
- Dosage Growth Defect: 10
- Dosage Lethality: 1
- Dosage Rescue: 9
- Negative Genetic: 250
- Phenotypic Enhancement: 35
- Phenotypic Suppression: 41
- Positive Genetic: 51
- Synthetic Growth Defect: 115
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 15
- Synthetic Rescue: 62
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-10-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.