EDE1 / YBL047C Overview
- Standard Name
- EDE1 1
- Systematic Name
- YBL047C
- SGD ID
- SGD:S000000143
- Aliases
- BUD15 8
- Feature Type
- ORF , Verified
- Description
- Endocytic adaptor; K63-specific Ub-chain binding protein involved in receptor endocytosis; scaffold protein that localizes to nascent endocytic sites after homo-oligomerization and organizes the endocytic machinery; role in autophagy of aberrant clathrin-mediated endocytosis protein condensates; binds membranes in a Ub-dependent manner; interacts with and functions upstream of Cmk2p to suppress stress-induced non-apoptotic cell death; putative cytokinesis regulator; homolog of mammalian Eps15 1 2 3 4 5 6 7
- Name Description
- EH Domains and Endocytosis 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ede1p is 1381 amino acids long, longer-lived, moderate in abundance; contains 3 EH domains, 2 EF-hand domains, and 1 ubiquitin-associated domain
- Length (a.a.)
- 1381
- Mol. Weight (Da)
- 150701.5
- Isoelectric Point
- 4.34
- Median Abundance (molecules/cell)
- 12983 +/- 3892
- Half-life (hr)
- 11.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all EDE1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Involved in organization of endocytic sites and in the regulation of cytokinesis; binds ubiquitin; localizes to actin cortical patches, bud neck, bud tip, cytoplasm, and shmoo tip
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin binding (IDA)
Biological Process
- Manually Curated
- involved in actin cortical patch organization (IMP)
- involved in endocytosis (IMP)
- involved in endoplasmic reticulum unfolded protein response (IMP)
- involved in positive regulation of cytokinesis (IGI)
- involved in regulation of protein localization (IMP)
Cellular Component
- Manually Curated
- located in actin cortical patch (IDA)
- located in cellular bud neck (IDA, HDA)
- located in cellular bud tip (IDA)
- located in cytoplasm (IDA)
- located in mating projection tip (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays small defect in vacuolar fragmentation, defective endocytosis, abnormal cell shape, sensitivity to visible light, increased resistance to hydroxyurea and to killer toxins; null is sensitive to bleomycin, fluconazole, fenpropimorph, caffeine, rapamycin, Calcofluor White, mercaptoethanol; overexpression confers resistance to ethanol
Classical Genetics
- null
- budding pattern: abnormal
- cell shape: abnormal
- chemical compound accumulation: increased
- endocytosis: decreased
- filamentous growth: abnormal
- metal resistance: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- viable
- overexpression
- conditional
- reduction of function
- null
- auxotrophy
- budding pattern: abnormal
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chitin deposition: increased
- competitive fitness: decreased
- competitive fitness: increased
- endocytosis: decreased
- heat sensitivity: increased
- killer toxin resistance: increased
- metal resistance: decreased
- metal resistance: increased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased
- stress resistance: decreased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Ede1p interacts physically with proteins involved in cytoskeleton organization; EDE1 interacts genetically with genes involved in protein targeting
589 total interactions for 375 unique genes
Physical Interactions
- Affinity Capture-MS: 44
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 8
- Biochemical Activity: 9
- Co-crystal Structure: 2
- Co-localization: 3
- FRET: 1
- PCA: 3
- Protein-RNA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 12
- Two-hybrid: 18
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 279
- Phenotypic Enhancement: 115
- Phenotypic Suppression: 12
- Positive Genetic: 35
- Synthetic Growth Defect: 18
- Synthetic Lethality: 12
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2004-03-28
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.