SRV2 / YNL138W Overview
- Standard Name
- SRV2 1
- Systematic Name
- YNL138W
- SGD ID
- SGD:S000005082
- Aliases
- CAP 7
- Feature Type
- ORF , Verified
- Description
- Cyclase-associated protein, CAP; N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis; N- and C-termini can function as physically separate proteins; mCAP1 is the mouse homolog 1 2 3 4 5 6
- Name Description
- Suppressor of RasVal19 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 526
- Mol. Weight (Da)
- 57514.0
- Isoelectric Point
- 5.31
- Median Abundance (molecules/cell)
- 8760 +/- 4920
- Half-life (hr)
- 10.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SRV2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Adenylate cyclase-binding protein involved in Ras protein signal transduction and actin filament organization; localizes to actin cortical patches
View computational annotations
Molecular Function
- Manually Curated
- enables actin binding (IDA)
- enables adenylate cyclase binding (IDA)
Biological Process
- Manually Curated
- involved in actin filament depolymerization (IDA)
- involved in actin filament organization (IDA, IMP)
- involved in actin filament severing (IDA)
- involved in positive regulation of actin filament depolymerization (IDA)
- involved in positive regulation of mitochondrial fission (IMP)
- involved in Ras protein signal transduction (IMP)
- involved in regulation of actin filament polymerization (IMP)
Cellular Component
- Manually Curated
- located in actin cortical patch (IDA)
- located in mating projection tip (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant exhibits slow growth, an abnormal actin cytoskeleton, and reduced localization of proteins to the bud tip; in large-scale studies, null mutant fails to respire or to mate, has abnormally large cells with elongated buds and abnormal lipid particle morphology, is sensitive to hyperosmotic and oxidative stress, and is resistant to K28 killer toxin and to manganese; conditional mutant exhibits chromosome instability at semi-permissive temperature; overexpression in the Sigma1278b genetic background causes greatly increased filamentous growth; null mutant exhibits filamentous growth in response to HU treatment; diploid heterozygous null mutant is sensitive to starvation and heat; null mutant is sensitive to a variety of chemicals
Classical Genetics
- null
- bud morphology: abnormal
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chitin deposition: normal
- competitive fitness: decreased
- haploproficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: increased
- lipid particle morphology: abnormal
- mating response: absent
- metal resistance: increased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- sporulation: decreased
- starvation resistance: decreased
- transposable element transposition: increased
- vegetative growth: decreased rate
- viable
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SRV2 is homologous to human CAP1, and has been used to study epithelial ovarian cancer (EOC) with findings suggesting that downregulation of CAP1 in tumor cells may be a therapeutic target for the treatment of patients with EOC
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
900 total interactions for 706 unique genes
Physical Interactions
- Affinity Capture-MS: 77
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 7
- Biochemical Activity: 1
- Co-fractionation: 3
- Co-localization: 2
- Co-purification: 1
- PCA: 14
- Protein-peptide: 2
- Reconstituted Complex: 11
- Two-hybrid: 15
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 2
- Negative Genetic: 606
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 4
- Positive Genetic: 116
- Synthetic Growth Defect: 9
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 15
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.