NRM1 / YNR009W Overview
- Standard Name
- NRM1 1
- Systematic Name
- YNR009W
- SGD ID
- SGD:S000005292
- Feature Type
- ORF , Verified
- Description
- Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase 1
- Name Description
- Negative Regulator of MBF targets 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 249
- Mol. Weight (Da)
- 27585.8
- Isoelectric Point
- 10.68
- Median Abundance (molecules/cell)
- 936 +/- 751
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NRM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription repressor subunit of MBF transcription complex involved in transcription regulation during G1/S cell cycle transition; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor binding (IPI)
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
77 total interactions for 63 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 6
- Biochemical Activity: 4
- Co-purification: 1
- Protein-RNA: 1
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 32
- Positive Genetic: 16
- Synthetic Growth Defect: 1
- Synthetic Lethality: 1
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Nrm1p is a repressor component of the MBF transcription complex, which is involved in regulation of transcription during the G1/S transition of the cell cycle. NRM1 itself is regulated by MBF and is expressed during late G1 phase. Nrm1p acts only after G1/S transcription is strongly activated, leading to Nrm1p accumulation. Nrm1p binds to the carboxy terminus of Swi6p at MBF target promoters via its GTB-containing domain, reestablishing MBF as a transcriptional repressor, and terminating expression of MBF target genes (including NRM1) by negative feedback as cells exit G1 phase. Rad53p can directly phosphorylate Nrm1p, preventing its association with MBF at target promoters, allowing expression of MBF targets. As cells exit mitosis, Nrm1p is targeted for degradation by the APC-Cdh1 ubiquitin ligase.
- Regulators
- 11
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.