Reference: Longhese MP, et al. (1994) Replication factor A is required in vivo for DNA replication, repair, and recombination. Mol Cell Biol 14(12):7884-90

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Abstract


Replication factor A (RF-A) is a heterotrimeric single-stranded-DNA-binding protein which is conserved in all eukaryotes. Since the availability of conditional mutants is an essential step to define functions and interactions of RF-A in vivo, we have produced and characterized mutations in the RFA1 gene, encoding the p70 subunit of the complex in Saccharomyces cerevisiae. This analysis provides the first in vivo evidence that RF-A function is critical not only for DNA replication but also for efficient DNA repair and recombination. Moreover, genetic evidence indicate that p70 interacts both with the DNA polymerase alpha-primase complex and with DNA polymerase delta.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Longhese MP, Plevani P, Lucchini G
Primary Lit For
RFA1 | RFA3 | RFA2 | DNA polymerase alpha:primase complex | DNA polymerase delta complex
Additional Lit For
rfa1-m2 | rfa1-M4

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