Reference: Kabeya Y, et al. (2005) Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy. Mol Biol Cell 16(5):2544-53

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Abstract


In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes are involved in not only the process of degradative autophagy, but also a biosynthetic process, the cytoplasm to vacuole (Cvt) pathway. In contrast, the ATG17 gene is required specifically in autophagy. To better understand the function of Atg17, we have performed a biochemical characterization of the Atg17 protein. We found that the atg17delta mutant under starvation condition was largely impaired in autophagosome formation and only rarely contained small autophagosomes, whose size was less than one-half of normal autophagosomes in diameter. Two-hybrid analyses and coimmunoprecipitation experiments demonstrated that Atg17 physically associates with Atg1-Atg13 complex, and this binding was enhanced under starvation conditions. Atg17-Atg1 binding was not detected in atg13delta mutant cells, suggesting that Atg17 interacts with Atg1 through Atg13. A point mutant of Atg17, Atg17(C24R), showed reduced affinity for Atg13, resulting in impaired Atg1 kinase activity and significant defects in autophagy. Taken together, these results indicate that Atg17-Atg13 complex formation plays an important role in normal autophagosome formation via binding to and activating the Atg1 kinase.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Kabeya Y, Kamada Y, Baba M, Takikawa H, Sasaki M, Ohsumi Y
Primary Lit For
ATG1 | ATG13 | ATG17 | ATG1/ULK1 protein kinase complex

Gene Ontology Annotations 5 entries for 3 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
ATG1part ofAtg1/ULK1 kinase complexIPI with ATG13SGD2017-11-28
ATG17part ofAtg1/ULK1 kinase complexIPI with ATG1SGD2017-11-28
ATG17part ofAtg1/ULK1 kinase complexIPI with ATG13SGD2017-11-28
ATG17involved inautophagosome assemblyIMPSGD2013-08-07
ATG13part ofAtg1/ULK1 kinase complexIPI with ATG1SGD2017-11-28
Showing 1 to 5 of 5 entries

Phenotype Annotations 2 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
ATG17autophagy: absent
classical geneticsnull
Allele: atg17-Δ
OtherMedia: nitrogen starvation + 1 mM PMSF
Details: autophagosomes not observed
ATG17viability: decreased
classical geneticsnull
Allele: atg17-Δ
OtherMedia: nitrogen starvation
Showing 1 to 2 of 2 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 3 entries for 3 genes

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InteractorInteractorAssayAnnotationActionModification
ATG1ATG13Affinity Capture-Westernmanually curatedBait-HitNo Modification
ATG1ATG17Affinity Capture-Westernmanually curatedBait-HitNo Modification
ATG17ATG13Affinity Capture-Westernmanually curatedBait-HitNo Modification
Showing 1 to 3 of 3 entries