ATG1 / YGL180W Overview


Standard Name
ATG1 1
Systematic Name
YGL180W
SGD ID
SGD:S000003148
Aliases
APG1 29 , CVT10 10 , AUT3 30
Feature Type
ORF , Verified
Description
Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; forms a complex with Atg13p and Atg17p during autophagy; required for re-phosphorylation of Atg13p during termination of autophagy, following prolonged nitrogen starvation; essential for cell cycle progression from G2/M to G1 under nitrogen starvation 2 3 4 5 6 7 8
Name Description
AuTophaGy related 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
ATG1 is located on the left arm of chromosome VII between GTS1 and replication origin ARS707; coding sequence is 2694 nucleotides long with 3 SNPs, 1 of which causes an amino acid polymorphism
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Atg1p is 897 amino acids long, very long-lived, and low in abundance; has 2 kinase domains; phosphorylated on 46 residues
Length (a.a.)
897
Mol. Weight (Da)
101707.3
Isoelectric Point
6.61
Median Abundance (molecules/cell)
1588 +/- 869
Half-life (hr)
33.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all ATG1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Protein serine/threonine kinase subunit of the Atg1p signalling complex involved in autophagy and cytoplasm-to-vacuole targeting (CVT) pathways

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows no autophagy, mitophagy or pexophagy, decreased chronological and replicative lifespan, increased sensitivity to nitrogen starvation and is unable to sporulate

Classical Genetics

null
overexpression
reduction of function
conditional
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast ATG1 is homologous to human ULK1, and has been used to study triple-receptor negative breast cancer
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Atg1p interacts physically with proteins involved in protein targeting; ATG1 interacts genetically with genes involved in protein targeting

955 total interactions for 637 unique genes

Physical Interactions

  • Affinity Capture-MS: 49
  • Affinity Capture-RNA: 12
  • Affinity Capture-Western: 57
  • Biochemical Activity: 248
  • Co-fractionation: 1
  • Co-localization: 3
  • Co-purification: 9
  • PCA: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 14
  • Two-hybrid: 32

Genetic Interactions

  • Dosage Growth Defect: 26
  • Dosage Rescue: 4
  • Negative Genetic: 387
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 16
  • Positive Genetic: 60
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 6
  • Synthetic Rescue: 8
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
ATG1 transcription is downregulated by Fyv5p and Spt10p in response to nitrogen starvation, and upregulated by Gcn4p and Sfl1p in response to nitrogen starvation; ATG1 transcription is downregulated by Rph1p in response to nutrient levels; Atg1p activity is upregulated by Snf1p in response to glucose starvation, and by Ptc2p and Ptc3p in response to MMS or rapamycin
Regulators
19
Targets
5
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2008-04-25

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
198
Additional
187
Reviews
221

Resources