Reference: Pessina S, et al. (2010)
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Abstract
The Saccharomyces cerevisiae Snf1 protein kinase has been reported to be required for adaptation to glucose limitation and for growth on non-fermentable carbon sources. Here we present novel findings indicating that Snf1, the key regulator of cellular energy, is also involved in yeast cell cycle control. The lack of Snf1 α-catalytic subunit down-regulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G 1/S transition, in cells grown in 2%, but not in 5% glucose. A non-phosphorylatable Snf1 rescues the slow growth phenotype, whereas a wild type or a phosphomimetic mutant is required to rescue growth rate and the G 1/S delay. Using either Snf1 or Swi6 as a bait, a specific interaction of Snf1 with Swi6, the regulatory subunit of MBF, was detected. In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G 1 to S transition, differing from the reported AMPK role in controlling the G 1/S transition in multicellular eukaryotes.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Pessina S,
Tsiarentsyeva V,
Busnelli S,
Vanoni M,
Alberghina L,
Coccetti P
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- REG1 | SNF4 | CLB5 | SNF1 | MBP transcription complex
- CDC28 | SLD2 | CLN1 | SWI6 | CLN2 | SIC1
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
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Reference |
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
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Reference |
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Disease Ontology Term |
Disease Ontology Term ID |
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Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
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Regulation Of |
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Post-translational Modifications
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Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
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SGA score |
P-value |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| SNF1 | SWI6 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| SNF1 | SWI6 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
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Complement ID |
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Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |