Reference: Pessina S, et al. (2010) Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast. Cell Cycle 9(11):2189-200

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Abstract


The Saccharomyces cerevisiae Snf1 protein kinase has been reported to be required for adaptation to glucose limitation and for growth on non-fermentable carbon sources. Here we present novel findings indicating that Snf1, the key regulator of cellular energy, is also involved in yeast cell cycle control. The lack of Snf1 α-catalytic subunit down-regulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G 1/S transition, in cells grown in 2%, but not in 5% glucose. A non-phosphorylatable Snf1 rescues the slow growth phenotype, whereas a wild type or a phosphomimetic mutant is required to rescue growth rate and the G 1/S delay. Using either Snf1 or Swi6 as a bait, a specific interaction of Snf1 with Swi6, the regulatory subunit of MBF, was detected. In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G 1 to S transition, differing from the reported AMPK role in controlling the G 1/S transition in multicellular eukaryotes.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Pessina S, Tsiarentsyeva V, Busnelli S, Vanoni M, Alberghina L, Coccetti P
Primary Lit For
REG1 | SNF4 | CLB5 | SNF1 | MBP transcription complex
Additional Lit For
CDC28 | SLD2 | CLN1 | SWI6 | CLN2 | SIC1

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 2 entries for 2 genes

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

InteractorInteractorAssayAnnotationActionModification
SNF1SWI6Affinity Capture-Westernmanually curatedBait-HitNo Modification
SNF1SWI6Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 2 of 2 entries