Primary Literature
TEXT HERE
- Absmeier E and Wahl MC (2021) Characterization of the Brr2 RNA Helicase and Its Regulation by Other Spliceosomal Proteins Using Gel-Based U4/U6 Di-snRNA Binding and Unwinding Assays. Methods Mol Biol 2209:193-215 PMID: 33201471
- Gautam A and Beggs JD (2019) Mutagenesis of Snu114 domain IV identifies a developmental role in meiotic splicing. RNA Biol 16(2):185-195 PMID: 30672374
- Henning LM, et al. (2017) A new role for FBP21 as regulator of Brr2 helicase activity. Nucleic Acids Res 45(13):7922-7937 PMID: 28838205
- Absmeier E, et al. (2016) Functions and regulation of the Brr2 RNA helicase during splicing. Cell Cycle 15(24):3362-3377 PMID: 27792457
- Galej WP, et al. (2016) Cryo-EM structure of the spliceosome immediately after branching. Nature 537(7619):197-201 PMID: 27459055
- Ledoux S and Guthrie C (2016) Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. J Biol Chem 291(23):11954-65 PMID: 27072132
- Absmeier E, et al. (2015) The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation. Genes Dev 29(24):2576-87 PMID: 26637280
- Burke JE, et al. (2015) Spliceosome assembly in the absence of stable U4/U6 RNA pairing. RNA 21(5):923-34 PMID: 25762536
- Hardin JW, et al. (2015) Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET. Nucleic Acids Res 43(22):10963-74 PMID: 26503251
- Liu S, et al. (2015) A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing. Elife 4:e07320 PMID: 26161500
- Rigo N, et al. (2015) Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex. EMBO J 34(24):3059-73 PMID: 26582754
- Santos K, et al. (2015) Crystallization and biochemical characterization of the human spliceosomal Aar2-Prp8(RNaseH) complex. Acta Crystallogr F Struct Biol Commun 71(Pt 11):1421-8 PMID: 26527271
- Zhang L, et al. (2015) Brr2 plays a role in spliceosomal activation in addition to U4/U6 unwinding. Nucleic Acids Res 43(6):3286-97 PMID: 25670679
- Long L, et al. (2014) The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem 289(13):8916-30 PMID: 24526689
- Mozaffari-Jovin S, et al. (2013) Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8. Science 341(6141):80-4 PMID: 23704370
- Nguyen TH, et al. (2013) Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site. Structure 21(6):910-19 PMID: 23727230
- Shcherbakova I, et al. (2013) Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy. Cell Rep 5(1):151-65 PMID: 24075986
- Weber G, et al. (2013) Structural basis for dual roles of Aar2p in U5 snRNP assembly. Genes Dev 27(5):525-40 PMID: 23442228
- Chen HC and Cheng SC (2012) Functional roles of protein splicing factors. Biosci Rep 32(4):345-59 PMID: 22762203
- Coltri PP and Oliveira CC (2012) Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. PLoS One 7(9):e45678 PMID: 23029180
- Düring L, et al. (2012) MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS One 7(9):e44373 PMID: 23028530
- Hahn D, et al. (2012) Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning. Genes Dev 26(21):2408-21 PMID: 23124065
- Hayduk AJ, et al. (2012) In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3' stem-loop. RNA 18(5):1075-90 PMID: 22411955
- Mozaffari-Jovin S, et al. (2012) The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA. Genes Dev 26(21):2422-34 PMID: 23124066
- Shchepachev V, et al. (2012) Mpn1, mutated in poikiloderma with neutropenia protein 1, is a conserved 3'-to-5' RNA exonuclease processing U6 small nuclear RNA. Cell Rep 2(4):855-65 PMID: 23022480
- Martin-Tumasz S, et al. (2011) A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop. Nucleic Acids Res 39(17):7837-47 PMID: 21653550
- Fabrizio P, et al. (2009) The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. Mol Cell 36(4):593-608 PMID: 19941820
- Frazer LN, et al. (2009) Analysis of synthetic lethality reveals genetic interactions between the GTPase Snu114p and snRNAs in the catalytic core of the Saccharomyces cerevisiae spliceosome. Genetics 183(2):497-515-1SI-4SI PMID: 19620389
- Maeder C, et al. (2009) ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8. Nat Struct Mol Biol 16(1):42-8 PMID: 19098916
- McGrail JC, et al. (2009) The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing. Nucleic Acids Res 37(13):4205-17 PMID: 19435883
- Pena V, et al. (2009) Common design principles in the spliceosomal RNA helicase Brr2 and in the Hel308 DNA helicase. Mol Cell 35(4):454-66 PMID: 19716790
- Zhang L, et al. (2009) Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2. Nat Struct Mol Biol 16(7):731-9 PMID: 19525970
- Zhao C, et al. (2009) Autosomal-dominant retinitis pigmentosa caused by a mutation in SNRNP200, a gene required for unwinding of U4/U6 snRNAs. Am J Hum Genet 85(5):617-27 PMID: 19878916
- Bellare P, et al. (2008) A role for ubiquitin in the spliceosome assembly pathway. Nat Struct Mol Biol 15(5):444-51 PMID: 18425143
- Häcker I, et al. (2008) Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol 15(11):1206-12 PMID: 18953335
- Karaduman R, et al. (2008) Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy. RNA 14(12):2528-37 PMID: 18971323
- Bae E, et al. (2007) Structure and interactions of the first three RNA recognition motifs of splicing factor prp24. J Mol Biol 367(5):1447-58 PMID: 17320109
- McManus CJ, et al. (2007) A dynamic bulge in the U6 RNA internal stem-loop functions in spliceosome assembly and activation. RNA 13(12):2252-65 PMID: 17925343
- Bellare P, et al. (2006) Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA 12(2):292-302 PMID: 16428608
- Boon KL, et al. (2006) Yeast ntr1/spp382 mediates prp43 function in postspliceosomes. Mol Cell Biol 26(16):6016-23 PMID: 16880513
- Cao S and Chen SJ (2006) Free energy landscapes of RNA/RNA complexes: with applications to snRNA complexes in spliceosomes. J Mol Biol 357(1):292-312 PMID: 16413034
- Chen CH, et al. (2006) Functional links between the Prp19-associated complex, U4/U6 biogenesis, and spliceosome recycling. RNA 12(5):765-74 PMID: 16540691
- Karaduman R, et al. (2006) RNA structure and RNA-protein interactions in purified yeast U6 snRNPs. J Mol Biol 356(5):1248-62 PMID: 16410014
- Small EC, et al. (2006) The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase. Mol Cell 23(3):389-99 PMID: 16885028
- Tardiff DF and Rosbash M (2006) Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly. RNA 12(6):968-79 PMID: 16618970
- Bottner CA, et al. (2005) Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe. Curr Genet 48(3):151-61 PMID: 16133344
- Lacadie SA and Rosbash M (2005) Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast. Mol Cell 19(1):65-75 PMID: 15989965
- Charron C, et al. (2004) The archaeal sRNA binding protein L7Ae has a 3D structure very similar to that of its eukaryal counterpart while having a broader RNA-binding specificity. J Mol Biol 342(3):757-73 PMID: 15342235
- Hilliker AK and Staley JP (2004) Multiple functions for the invariant AGC triad of U6 snRNA. RNA 10(6):921-8 PMID: 15146076
- Bartels C, et al. (2002) The ribosomal translocase homologue Snu114p is involved in unwinding U4/U6 RNA during activation of the spliceosome. EMBO Rep 3(9):875-80 PMID: 12189173
- Bell M, et al. (2002) p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor. EMBO J 21(11):2724-35 PMID: 12032085
- Kuhn AN, et al. (2002) Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activation. Proc Natl Acad Sci U S A 99(14):9145-9 PMID: 12087126
- Makarova OV, et al. (2002) Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing. EMBO J 21(5):1148-57 PMID: 11867543
- Rader SD and Guthrie C (2002) A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation. RNA 8(11):1378-92 PMID: 12458792
- Stevens SW, et al. (2002) Composition and functional characterization of the yeast spliceosomal penta-snRNP. Mol Cell 9(1):31-44 PMID: 11804584
- Stark H, et al. (2001) Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle. Nature 409(6819):539-42 PMID: 11206553
- Stevens SW, et al. (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53 PMID: 11720284
- Walke S, et al. (2001) Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain. J Mol Biol 308(1):49-58 PMID: 11302706
- Xu D and Friesen JD (2001) Splicing factor slt11p and its involvement in formation of U2/U6 helix II in activation of the yeast spliceosome. Mol Cell Biol 21(4):1011-23 PMID: 11158289
- Fromont-Racine M, et al. (2000) Genome-wide protein interaction screens reveal functional networks involving Sm-like proteins. Yeast 17(2):95-110 PMID: 10900456
- Kuhn AN and Brow DA (2000) Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation. Genetics 155(4):1667-82 PMID: 10924465
- Palfi Z, et al. (2000) The spliceosomal snRNP core complex of Trypanosoma brucei: cloning and functional analysis reveals seven Sm protein constituents. Proc Natl Acad Sci U S A 97(16):8967-72 PMID: 10900267
- Pereira V, et al. (2000) A gene associated with filamentous growth in Ophiostoma novo-ulmi has RNA-binding motifs and is similar to a yeast gene involved in mRNA splicing. Curr Genet 37(2):94-103 PMID: 10743565
- Reidt U, et al. (2000) Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin. J Biol Chem 275(11):7439-42 PMID: 10713041
- Xue D, et al. (2000) U snRNP assembly in yeast involves the La protein. EMBO J 19(7):1650-60 PMID: 10747032
- Kambach C, et al. (1999) Structure and assembly of the spliceosomal small nuclear ribonucleoprotein particles. Curr Opin Struct Biol 9(2):222-30 PMID: 10322216
- Kambach C, et al. (1999) Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs. Cell 96(3):375-87 PMID: 10025403
- Kim DH and Rossi JJ (1999) The first ATPase domain of the yeast 246-kDa protein is required for in vivo unwinding of the U4/U6 duplex. RNA 5(7):959-71 PMID: 10411139
- Kuhn AN, et al. (1999) Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome. Mol Cell 3(1):65-75 PMID: 10024880
- Lygerou Z, et al. (1999) A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. Mol Cell Biol 19(3):2008-20 PMID: 10022888
- Vidaver RM, et al. (1999) Multiple functions of Saccharomyces cerevisiae splicing protein Prp24 in U6 RNA structural rearrangements. Genetics 153(3):1205-18 PMID: 10545453
- Ayadi L, et al. (1998) Functional and structural characterization of the prp3 binding domain of the yeast prp4 splicing factor. J Mol Biol 284(3):673-87 PMID: 9826507
- Laggerbauer B, et al. (1998) The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro. Proc Natl Acad Sci U S A 95(8):4188-92 PMID: 9539711
- Raghunathan PL and Guthrie C (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr Biol 8(15):847-55 PMID: 9705931
- Raghunathan PL and Guthrie C (1998) A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles. Science 279(5352):857-60 PMID: 9452384
- Ayadi L, et al. (1997) Mutations within the yeast U4/U6 snRNP protein Prp4 affect a late stage of spliceosome assembly. RNA 3(2):197-209 PMID: 9042946
- Horowitz DS and Krainer AR (1997) A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor. Genes Dev 11(1):139-51 PMID: 9000057
- Horowitz DS, et al. (1997) A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs. RNA 3(12):1374-87 PMID: 9404889
- Urushiyama S, et al. (1997) The prp1+ gene required for pre-mRNA splicing in Schizosaccharomyces pombe encodes a protein that contains TPR motifs and is similar to Prp6p of budding yeast. Genetics 147(1):101-15 PMID: 9286671
- Weidenhammer EM, et al. (1997) Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae. Mol Cell Biol 17(7):3580-8 PMID: 9199293
- Cooper M, et al. (1995) Identification and characterization of Uss1p (Sdb23p): a novel U6 snRNA-associated protein with significant similarity to core proteins of small nuclear ribonucleoproteins. EMBO J 14(9):2066-75 PMID: 7744012
- Ghetti A, et al. (1995) Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs. RNA 1(2):132-45 PMID: 7585243
- Jandrositz A and Guthrie C (1995) Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs. EMBO J 14(4):820-32 PMID: 7882985
- Roy J, et al. (1995) Structurally related but functionally distinct yeast Sm D core small nuclear ribonucleoprotein particle proteins. Mol Cell Biol 15(1):445-55 PMID: 7799953
- Séraphin B (1995) Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs. EMBO J 14(9):2089-98 PMID: 7744014
- Hu J, et al. (1994) Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions. Nucleic Acids Res 22(9):1724-34 PMID: 8202378
- Lehmeier T, et al. (1994) cDNA cloning of the Sm proteins D2 and D3 from human small nuclear ribonucleoproteins: evidence for a direct D1-D2 interaction. Proc Natl Acad Sci U S A 91(25):12317-21 PMID: 7527560
- Maddock JR, et al. (1994) Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics 136(3):833-47 PMID: 8005438
- Tarn WY, et al. (1993) Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA. Proc Natl Acad Sci U S A 90(22):10821-5 PMID: 8248176
- Blanton S, et al. (1992) PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels. Mol Cell Biol 12(9):3939-47 PMID: 1508195
- Elliott DJ, et al. (1992) A yeast splicing factor is localized in discrete subnuclear domains. EMBO J 11(10):3731-6 PMID: 1396568
- Vankan P, et al. (1992) Roles of U4 and U6 snRNAs in the assembly of splicing complexes. EMBO J 11(1):335-43 PMID: 1740112
- Jamieson DJ, et al. (1991) A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature 349(6311):715-7 PMID: 1996139
- Séraphin B, et al. (1991) Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly. Nucleic Acids Res 19(14):3857-60 PMID: 1830649
- Shannon KW and Guthrie C (1991) Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs. Genes Dev 5(5):773-85 PMID: 1827420
- Strauss EJ and Guthrie C (1991) A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes Dev 5(4):629-41 PMID: 2010088
- Yean SL and Lin RJ (1991) U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction. Mol Cell Biol 11(11):5571-7 PMID: 1833635
- Abovich N, et al. (1990) The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding. Mol Cell Biol 10(12):6417-25 PMID: 2147224
- Bordonné R, et al. (1990) Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo. Genes Dev 4(7):1185-96 PMID: 2145195
- Hausner TP, et al. (1990) Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles. Genes Dev 4(12A):2146-56 PMID: 2176635
- Banroques J and Abelson JN (1989) PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle. Mol Cell Biol 9(9):3710-9 PMID: 2528687
- Bjørn SP, et al. (1989) PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle. Mol Cell Biol 9(9):3698-709 PMID: 2528686