CSE4 / YKL049C Overview
- Standard Name
- CSE4 1
- Systematic Name
- YKL049C
- SGD ID
- SGD:S000001532
- Aliases
- CSL2 14
- Feature Type
- ORF , Verified
- Description
- Centromeric histone H3-like protein; associates with promoters, accessible chromatin and RNAPII-bound regions; centromeric association promotes kinetochore assembly and Sgo1p association with CEN chromatin; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting biorientation; phosphorylation by Cdc7p maintains Cse4p at kinetochores and promotes chromosome segregation; R37 methylation regulates kinetochore function and chromosome segregation; complements human homolog CENPA 1 2 3 4 5 6 7 8 9 10 11 12 13
- Name Description
- Chromosome SEgregation 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CSE4/YKL049C is located on the left arm of chromosome XI between LPX2 peroxisomal lipase and ELM1 protein kinase; coding sequence is 690 nucleotides long with one SNP that causes an Ala/Gly polymorphism at residue 167
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cse4p is 229 amino acids long, low in abundance; contains 4 histone H3/CENP-A motifs; methylated on R37, acetylated on K49, sumoylated on 4 lysines, ubiquitinylated on 5 lysines, phosphorylated by Cdc5p on 9 serines
- Length (a.a.)
- 229
- Mol. Weight (Da)
- 26846.8
- Isoelectric Point
- 9.78
- Median Abundance (molecules/cell)
- 2937 +/- 1414
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CSE4 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding protein that interacts with centromeres and the centromere-like sequence of the 2-micron circle; involved in kinetochore assembly, sister chromatid segregation, and 2-micron circle partitioning; localizes to the centromere-specific nucleosome, the centromeric region and the circular 2 micron plasmid DNA
View computational annotations
Molecular Function
- Manually Curated
- enables centromeric DNA binding (IDA)
- enables sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in 2-micrometer plasmid partitioning (IMP)
- involved in kinetochore assembly (IMP)
- involved in mitotic cell cycle (IDA)
- involved in mitotic sister chromatid segregation (IMP)
- involved in protein localization to CENP-A containing chromatin (IMP)
Cellular Component
- Manually Curated
- located in 2-micrometer circle DNA (IDA)
- part of CENP-A containing nucleosome (IDA)
- located in condensed chromosome, centromeric region (IDA, IGI)
- is active in kinetochore (IDA)
- located in peroxisome (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; downregulation of expression causes abnormal chromosome segregation; conditional mutants display reduced levels of centromere-localized proteins at the restrictive temperature; in large-scale studies, overexpression causes decreased growth rate; heterozygous null mutant is haploinsufficient
Classical Genetics
- null
- repressible
- reduction of function
- conditional
- unspecified
- null
- overexpression
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CSE4 is homologous to human H2BW1, and has been used to study mutations found in patients with oligospermia and male infertility; yeast CSE4 is also homologous to human CENP-A and has been used to study mutations found in patients with various cancers
High-Throughput
- is_implicated_in male infertility (IGI, ISS)
- is_implicated_in oligospermia (IGI, ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cse4p interacts physically with proteins involved in mitotic cell cycle and transcription; CSE4 interacts genetically with genes involved in transcription and mitotic cell cycle
1875 total interactions for 1311 unique genes
Physical Interactions
- Affinity Capture-MS: 340
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 83
- Biochemical Activity: 9
- Co-crystal Structure: 3
- Co-localization: 12
- Co-purification: 39
- FRET: 1
- PCA: 2
- Protein-peptide: 2
- Reconstituted Complex: 27
- Two-hybrid: 18
Genetic Interactions
- Dosage Growth Defect: 6
- Dosage Lethality: 34
- Dosage Rescue: 17
- Negative Genetic: 972
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 11
- Positive Genetic: 199
- Synthetic Growth Defect: 62
- Synthetic Lethality: 20
- Synthetic Rescue: 15
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CSE4 promoter is bound by Stp1p; CSE4 transcription is regulated by Sfp1p; Cse4 protein activity is downregulated by Cdc4p and Met30p during mitotic G1, S, and M phases; Cse4 protein activity is upregulated by Cdc5p during mitotic metaphase
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.