ORC1 / YML065W Overview
- Standard Name
- ORC1 1
- Systematic Name
- YML065W
- SGD ID
- SGD:S000004530
- Feature Type
- ORF , Verified
- Description
- Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; role in chromatin organization at replication origins, along with chromatin remodellers; ORC1 has a paralog, SIR3, that arose from the whole genome duplication 2 3 5 6
- Name Description
- Origin Recognition Complex 4
- Paralog
- SIR3 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- ORC1/YML065W is located on the left arm of chromosome XIII between SMA2 meiosis-specific prospore membrane protein and TEM1 GTPase; coding sequence is 2745 nucleotides long with 21 SNPs, 5 of which cause amino acid polymorphisms, and an in-frame 12-nucleotide insertion in strains Y55 and CEN.PK in a repetitive section of the gene that adds the 4 amino acids EDED after residue 287; ORC1 has a paralog, SIR3, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Orc1p is 914 amino acids long, extremely short-lived, low in abundance; contains two disordered regions; acetylated on K719, phosphorylated on S237 and S442, sumoylated on 12 lysines
- Length (a.a.)
- 914
- Mol. Weight (Da)
- 104384.2
- Isoelectric Point
- 5.35
- Median Abundance (molecules/cell)
- 1449 +/- 585
- Half-life (min)
- 34.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ORC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP binding protein involved in DNA replication initiation, maintenance of rDNA and silent mating-type cassette heterochromatin formation; localizes to nucleus; subunit of DNA replication pre-initiation complex
View computational annotations
Molecular Function
- Manually Curated
- enables ATP binding (IDA, IMP)
- enables ATP hydrolysis activity (IMP)
- enables chromatin binding (IDA)
- enables DNA replication origin binding (IDA)
- enables nucleosome binding (IDA)
Biological Process
- Manually Curated
- involved in DNA replication initiation (IMP)
- involved in maintenance of rDNA (IDA)
- acts upstream of or within nucleosome organization (IMP)
- involved in pre-replicative complex assembly involved in nuclear cell cycle DNA replication (IDA, IMP)
- involved in silent mating-type cassette heterochromatin formation (IDA, IGI)
Cellular Component
- Manually Curated
- part of DNA replication preinitiation complex (IDA)
- part of nuclear origin of replication recognition complex (IDA, IMP)
- part of nuclear pre-replicative complex (IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- ORC1/YML065W is an essential gene; null mutants are inviable. Reduction of function mutants exhibit delayed G1/S phase transition, decreased cell cycle progression in the S phase, decreased vegetative growth, and increased competitive fitness. Some reduction of function mutants are inviable. Repressible alleles exhibit abnormal cell cycle progression in the G1 phase. Overexpression of ORC1 results in decreased vegetative growth and reduced chromosome/plasmid maintenance. Conditional mutants show arrested cell cycle progression in the M phase, delayed G1/S phase transition, increased colony sectoring, increased heat sensitivity, decreased viability, abnormal chromosome/plasmid maintenance, and increased killer toxin resistance.
Classical Genetics
- reduction of function
- overexpression
- conditional
- null
- null
- repressible
- reduction of function
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast ORC1 is homologous to human ORC1, and has been used to study Meier-Gorlin syndrome (MGS), a form of dwarfism caused by recessive mutations in genes encoding components of the pre-replication complex
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Orc1p interacts physically with proteins involved in rRNA processing and translation; ORC1 interacts genetically with genes involved in DNA repair; the orc1 null mutant is inviable, the null mutant of paralog sir3 is viable, the orc1 sir3 double mutant has not been annotated for phenotype
889 total interactions for 671 unique genes
Physical Interactions
- Affinity Capture-MS: 362
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 43
- Biochemical Activity: 4
- Co-crystal Structure: 6
- Co-fractionation: 6
- Co-localization: 2
- Co-purification: 11
- Reconstituted Complex: 37
- Two-hybrid: 15
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 1
- Negative Genetic: 342
- Phenotypic Suppression: 1
- Positive Genetic: 39
- Synthetic Growth Defect: 6
- Synthetic Lethality: 3
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
No regulation data available.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-10-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.