FUN30 / YAL019W Overview
- Standard Name
- FUN30 1
- Systematic Name
- YAL019W
- SGD ID
- SGD:S000000017
- Feature Type
- ORF , Verified
- Description
- SWI/SNF-like ATP-dependent chromatin remodeler; involved in silencing at the MAT locus, telomeres and centromeres; enriched at centromeres and required to promote correct chromatin structure around centromeres and at the boundary element of silent mating type loci; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection; role in meiotic DSB resection and recombination; potential Cdc28p substrate 1 2 3 5 6 7 8 9 10
- Name Description
- Function Unknown Now 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- FUN30 is located on the left arm of chromosome I between ATS1 wobble nucleoside modification protein and LDS1 spore wall assembly protein; coding sequence is 3396 nucleotides long with 11 nonsynonymous SNPs, 30 synonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Fun30p is 1131 amino acids long, shorter-lived, low in abundance; has 1 helicase domain, 1 ATP-binding domain; phosphorylated on 36 residues
- Length (a.a.)
- 1131
- Mol. Weight (Da)
- 128470.9
- Isoelectric Point
- 5.04
- Median Abundance (molecules/cell)
- 3730 +/- 1192
- Half-life (hr)
- 7.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FUN30 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP-dependent chromatin remodeler; involved in DNA double-strand break processing and heterochromatin maintenence by silencing at telomeres, silent mating-type cassettes, and the rDNA; involved in meiotic DSB resection and recombination; localizes to centromeric regions of chromosomes
View computational annotations
Molecular Function
- Manually Curated
- enables ATP-dependent chromatin remodeler activity (IDA, IMP)
- enables chromatin binding (IDA)
- enables DNA binding (IDA)
Biological Process
- Manually Curated
- acts upstream of or within chromatin remodeling (IMP)
- involved in DNA double-strand break processing (IMP)
- involved in heterochromatin formation (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of RNA splicing (IMP)
- involved in rDNA heterochromatin formation (IMP)
- involved in silent mating-type cassette heterochromatin formation (IMP)
- involved in subtelomeric heterochromatin formation (IMP)
Cellular Component
- Manually Curated
- located in chromosome, centromeric region (IDA)
- located in mating-type region heterochromatin (IDA)
- located in mitochondrion (HDA)
- located in nucleus (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene, null mutant shows decreased silencing at telomeres, rDNA and siilent mating loci and also chromosome segregation defects;
Classical Genetics
- unspecified
- overexpression
- null
- null
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- heat sensitivity: decreased
- killer toxin resistance: increased
- metal resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- silencing: decreased
- UV resistance: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Fun30p interacts physically with proteins involved in DNA repair; FUN30 interacts genetically with genes involved in DNA repair
303 total interactions for 222 unique genes
Physical Interactions
- Affinity Capture-MS: 28
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 11
- Biochemical Activity: 1
- Far Western: 2
- Proximity Label-MS: 2
- Reconstituted Complex: 5
- Two-hybrid: 3
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 156
- Phenotypic Enhancement: 28
- Phenotypic Suppression: 7
- Positive Genetic: 25
- Synthetic Growth Defect: 17
- Synthetic Lethality: 4
- Synthetic Rescue: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.