SLA1 / YBL007C Overview
- Standard Name
- SLA1 1
- Systematic Name
- YBL007C
- SGD ID
- SGD:S000000103
- Feature Type
- ORF , Verified
- Description
- Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains with a PH-like domain amid them at residues 253-339 which confers lipid-binding properties 2 3 4 5 6
- Name Description
- Synthetic Lethal with ABP1 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SLA1/YBL007C is located on the left arm of chromosome II near the centromere between HIR1 nucleosome assembly complex subunit and LDB7 RSC chromatin remodeling complex subunit; coding sequence is 2523 nucleotides long with 37 SNPs, 15 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Sla1p is 1244 amino acids long, of average half-life, and low in abundance; contains disordered regions throughout, and has 3 SH3 domains with a PH-like domain amid them at residues 253-339 which confers lipid-binding properties; acetylated on K303 and K556, N-glycosylated on N322, ubiquitinylated on 20 residues, phosphorylated on 98 residues
- Length (a.a.)
- 1244
- Mol. Weight (Da)
- 135835.7
- Isoelectric Point
- 5.62
- Median Abundance (molecules/cell)
- 8674 +/- 2996
- Half-life (hr)
- 10.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SLA1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Endocytic adaptor protein that interacts with ubiquitin; involved in endocytosis and actin cortical patch assembly; inhibits Arp2/3-complex mediated actin nucleation; shuttles between the nucleus and actin cortical patches
View computational annotations
Molecular Function
- Manually Curated
- enables cargo adaptor activity (IMP)
- enables lipid binding (IDA)
- enables ubiquitin binding (IDA)
Biological Process
- Manually Curated
- involved in actin cortical patch assembly (IMP)
- involved in endocytosis (IMP)
- involved in negative regulation of Arp2/3 complex-mediated actin nucleation (IMP, IDA, IPI)
Cellular Component
- Manually Curated
- located in actin cortical patch (IDA)
- part of actin cytoskeleton-regulatory complex (IDA)
- located in cell cortex (IDA)
- located in cellular bud neck (HDA)
- located in mating projection tip (HDA)
- located in nucleus (IMP, IDA)
- part of SLAC complex (IDA, IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- SLA1/YBL007C is a non-essential gene; null mutant is slow-growing, viable, temperature sensitive, has decreased competitive fitness in various growth media and a respiratory growth defect; homozygous diploid null mutant has abnormally round cells, a random budding pattern, cell size heterogeneity, actin cytoskeleton defects that are more pronounced at elevated temperature, and defects in HU-induced filamentous growth; null mutant has an endocytotic defect, resulting reduced localization of Bud8p and Rax2p to growing bud tips; null mutant displays decreased resistance to hyperosmotic and oxidative stress, metals, acid pH, and has decreased thermotolerance; chitin deposition is increased at least two fold in the null mutant
Classical Genetics
- overexpression
- null
- actin cytoskeleton morphology: abnormal
- bipolar budding pattern: decreased
- bud morphology: abnormal
- cell shape: abnormal
- cell wall morphology: abnormal
- cellular morphology: abnormal
- chemical compound accumulation: increased
- chitin deposition: increased
- endocytosis: absent
- endocytosis: decreased
- filamentous growth: abnormal
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- metal resistance: decreased
- nuclear morphology: abnormal
- prion state: abnormal
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- small molecule transport: decreased
- vacuolar morphology: abnormal
- unspecified
- reduction of function
- null
- acid pH resistance: decreased
- bud morphology: abnormal
- budding pattern: abnormal
- cell shape: abnormal
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound excretion: increased
- chitin deposition: increased
- competitive fitness: decreased
- endocytosis: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- killer toxin resistance: increased
- metal resistance: decreased
- metal resistance: increased
- mitochondrial genome maintenance: abnormal
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased rate
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased rate
- viable
- unspecified
- conditional
- reduction of function
- acid pH resistance: increased
- alkaline pH resistance: increased
- heat sensitivity: increased
- metal resistance: decreased
- metal resistance: increased
- osmotic stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- starvation resistance: decreased
- starvation resistance: increased
- utilization of carbon source: decreased
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Sla1p interacts physically with proteins involved in cytoskeleton organization; SLA1 interacts genetically with genes involved in transcription
1301 total interactions for 725 unique genes
Physical Interactions
- Affinity Capture-MS: 76
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 24
- Biochemical Activity: 20
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 1
- FRET: 11
- PCA: 23
- Protein-peptide: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 18
- Two-hybrid: 141
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 6
- Dosage Rescue: 1
- Negative Genetic: 777
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 6
- Positive Genetic: 91
- Synthetic Growth Defect: 40
- Synthetic Lethality: 42
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-06-13
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.