HHT1 / YBR010W Overview
- Standard Name
- HHT1
- Systematic Name
- YBR010W
- SGD ID
- SGD:S000000214
- Aliases
- BUR5 20 , SIN2 21
- Feature Type
- ORF , Verified
- Description
- Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage 1 2 3 4 5 6 7 8 9
- Name Description
- Histone H Three
- Paralog
- HHT2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Histone H3 is 136 amino acids long, moderate in abundance; contains 7 histone H3/CENP-A domains; thiophosphorylated on S58, deamidated on 2 glutamines, propanoylated on 2 lysines, ethylated on 2 glutamines, butanoylated on 3 lysines, ubiquitinylated on 4 lysines, crotonylated on 5 lysines, acetylated on 8 lysines, methylated on 9 lysines, sumoylated on 11 lysines
- Length (a.a.)
- 136
- Mol. Weight (Da)
- 15378.3
- Isoelectric Point
- 11.99
- Median Abundance (molecules/cell)
- 13263 +/- 11969
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HHT1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Histone H1 is a protein that enables cupric reductase (NADH) activity and DNA binding. It plays a crucial role in various biological processes, including aerobic respiration, chromatin organization, global genome nucleotide-excision repair, intracellular copper ion homeostasis, rRNA transcription, and sexual sporulation resulting in the formation of a cellular spore. Histone H1 is a component of the CENP-A containing nucleosome, the nucleosome, and the replication fork protection complex, and it is located in the nucleus.
View computational annotations
Molecular Function
- Manually Curated
- enables cupric reductase (NADH) activity (IDA, IMP)
- enables DNA binding (TAS)
Biological Process
- Manually Curated
- involved in aerobic respiration (IMP)
- involved in chromatin organization (TAS)
- involved in global genome nucleotide-excision repair (IMP)
- involved in intracellular copper ion homeostasis (IMP)
- involved in rRNA transcription (IMP)
- involved in sexual sporulation resulting in formation of a cellular spore (IMP)
Cellular Component
- Manually Curated
- part of CENP-A containing nucleosome (IDA)
- part of nucleosome (TAS)
- located in nucleus (HDA)
- part of replication fork protection complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene, due to the presence of two identical genes, HHT1 and HHT2; conditional mutations in cells lacking HHT2 confer slow growth and sensitivity to 6-azauracil, benomyl, hydroxyurea and sirolimus (rapamycin)
Classical Genetics
- conditional
- reduction of function
- null
- null
- chemical compound accumulation: abnormal
- competitive fitness: decreased
- competitive fitness: increased
- heat sensitivity: increased
- metal resistance: decreased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- toxin resistance: increased
- transposable element transposition: increased
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Histone H1 interacts physically with proteins involved in transcription and chromatin organization; HHT1 interacts genetically with genes involved in transcription and chromatin organization
1876 total interactions for 935 unique genes
Physical Interactions
- Affinity Capture-Luminescence: 14
- Affinity Capture-MS: 653
- Affinity Capture-RNA: 15
- Affinity Capture-Western: 163
- Biochemical Activity: 73
- Co-crystal Structure: 13
- Co-localization: 10
- Co-purification: 9
- Cross-Linking-MS (XL-MS): 10
- Far Western: 2
- FRET: 6
- PCA: 3
- Protein-peptide: 31
- Proximity Label-MS: 2
- Reconstituted Complex: 157
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 7
- Dosage Lethality: 15
- Dosage Rescue: 5
- Negative Genetic: 202
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 48
- Positive Genetic: 34
- Synthetic Growth Defect: 300
- Synthetic Lethality: 39
- Synthetic Rescue: 53
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- HHT1/YBR010W is regulated by various proteins and complexes that influence its activity and transcription. Gcn5p, Ngg1p, Set1p, and Set2p act as protein modifiers, enhancing Hht1p protein activity. Chromatin modifiers Hst2p and Yta7p, and transcription factors Nhp6ap and Nhp6bp, negatively regulate HHT1 transcription. The SAGA complex, Spt10p, and Spt21p positively regulate HHT1 transcription. Cka1p and Sch9p positively regulate Hht1p protein activity in response to glucose starvation, and Gcn5p also promotes Hht1p activity during the G1/S transition of the mitotic cell cycle.
- Regulators
- 38
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-05-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.