HYP2 / YEL034W Overview
- Standard Name
- HYP2 1
- Systematic Name
- YEL034W
- SGD ID
- SGD:S000000760
- Aliases
- eIF5A , eIF-5A 11 , TIF51A 11
- Feature Type
- ORF , Verified
- Description
- Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has paralog ANB1 that arose from whole genome duplication; in humans EIF5A variants cause a disorder characterized by variable combinations of developmental delay, microcephaly, micrognathia and dysmorphism; human EIF5A complements inviability of yeast hyp2 anb1 double null mutant 2 3 4 5 6 7 8 9 10
- Name Description
- HYPusine-containing protein 1
- Paralog
- ANB1 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- HYP2 is located on the left arm of chromosome V between uncharacterized gene UTR5 and uncharacterized gene MTC7; dubious ORF YEL034C-A completely overlaps HYP2 on the opposite strand; coding sequence is 474 nucleotides long with 3 SNPs, one of which causes an Asp/Glu polymorphism at residue 122; HYP2 has paralog ANB1 from whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 157
- Mol. Weight (Da)
- 17103.4
- Isoelectric Point
- 4.56
- Median Abundance (molecules/cell)
- 50245 +/- 22813
- Half-life (hr)
- 9.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HYP2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytosolic ribosome-binding translation initiation factor; involved in positive regulation of cytoplasmic translational elongation through polyproline stretches
View computational annotations
Molecular Function
- Manually Curated
- enables ribosome binding (IDA)
- enables RNA binding (IDA)
- enables translation elongation factor activity (IDA, IMP)
- enables translation initiation factor activity (IDA)
Biological Process
- Manually Curated
- involved in positive regulation of cytoplasmic translational elongation through polyproline stretches (IMP)
- involved in positive regulation of translational elongation (IMP, IDA, IGI)
- involved in positive regulation of translational initiation (IDA)
- involved in positive regulation of translational termination (IDA)
- involved in translational frameshifting (IGI)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- located in mitochondrion (HDA)
- located in perinuclear region of cytoplasm (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in S288C; conditional (ts) mutations lead to accumulation of polysomes, abnormal actin cytoskeleton and defects in shmoo formation; null mutant is reported as viable but slow growing in some strain backgrounds
Classical Genetics
- null
- conditional
- null
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast HYP2 is homologous to human EIF5A and has been used to study how SUMOylation modulates eIF5A activities in pancreatic ductal adenocarcinoma cells
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The hyp2 null mutant is inviable in the S288C and W303 backgrounds, but viable in others; the null mutant of paralog anb1 is viable; the hyp2 anb1 double mutant is inviable.
1235 total interactions for 1084 unique genes
Physical Interactions
- Affinity Capture-MS: 130
- Affinity Capture-RNA: 14
- Affinity Capture-Western: 11
- Co-fractionation: 1
- Co-purification: 2
- Cross-Linking-MS (XL-MS): 2
- PCA: 3
- Protein-RNA: 1
- Proximity Label-MS: 3
- Reconstituted Complex: 2
- Two-hybrid: 4
Genetic Interactions
- Dosage Rescue: 30
- Negative Genetic: 639
- Phenotypic Suppression: 1
- Positive Genetic: 375
- Synthetic Growth Defect: 5
- Synthetic Lethality: 6
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 13
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2009-08-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.