GPA1 / YHR005C Overview
- Standard Name
- GPA1 1
- Systematic Name
- YHR005C
- SGD ID
- SGD:S000001047
- Aliases
- CDC70 26 , DAC1 27 , SCG1 8
- Feature Type
- ORF , Verified
- Description
- Subunit of G protein involved in pheromone response; GTP-binding alpha subunit of heterotrimeric G protein; negatively regulates mating pathway by sequestering G(beta)gamma and triggering an adaptive response; activates Vps34p at endosome; protein abundance increases in response to DNA replication stress; mutations in human homolog GNAS associated with McCune-Albright syndrome 2 3 4 5 6
- Name Description
- G Protein Alpha subunit 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Gpa1p is 472 amino acids long, short-lived, low in abundance; myristoylated on G2, palmitoylated on C3, ubiquitinylated on 5 lysines, phosphorylated on 8 residues; Gpa1 protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 472
- Mol. Weight (Da)
- 54077.2
- Isoelectric Point
- 7.7
- Median Abundance (molecules/cell)
- 5057 +/- 2074
- Half-life (hr)
- 6.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GPA1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Heterotrimeric G-protein complex GTPase involved in pheromone signaling and desensitization during mating; plays a role in mating-related nuclear migration and karyogamy; also involved in inositol lipid-mediated signaling, regulation of MAPK export from the nucleus, and the heterotrimeric G-protein complex cycle; localizes to the plasma membrane and endosome
View computational annotations
Molecular Function
- Manually Curated
- enables G-protein beta-subunit binding (IPI)
- enables GTPase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in karyogamy involved in conjugation with cellular fusion (IMP)
- involved in nuclear migration involved in conjugation with cellular fusion (IMP)
- involved in pheromone-dependent signal transduction involved in conjugation with cellular fusion (IGI, IMP)
- involved in regulation of carbohydrate metabolic process (IMP)
Cellular Component
- Manually Curated
- is active in cytosol (HDA)
- located in endosome (IDA)
- part of heterotrimeric G-protein complex (IPI, IMP, IDA)
- located in plasma membrane (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- GPA1/YHR005C is a non-essential gene; null mutants exhibit increased cell size, abnormal cell shape, and abnormal liquid culture appearance. They are viable with increased chronological lifespan and overall lifespan, but show absent respiratory growth, decreased sporulation efficiency, decreased starvation resistance, and decreased vegetative growth. Null mutants are impaired in mating response. Repressible mutants display arrested and abnormal cell cycle progression in the G1 phase, abnormal cell shape, and abnormal nuclear position. Reduction of function mutants show decreased competitive fitness and absence of pheromone-induced cell cycle arrest. Overexpression of GPA1 results in abnormal nuclear position and decreased pheromone sensitivity. Activation mutants exhibit arrested cell cycle progression, decreased protein transport, and decreased vegetative growth. Conditional mutants show arrested cell cycle progression in the G1 phase, increased heat sensitivity, decreased nuclear fusion during mating, abnormal nuclear position, abnormal protein/peptide distribution, and increased RNA accumulation.
Classical Genetics
- repressible
- reduction of function
- null
- overexpression
- activation
- conditional
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: increased
- haploinsufficient
- lifespan: increased
- liquid culture appearance: abnormal
- mating response: absent
- protein activity: increased
- resistance to chemicals: decreased
- respiratory growth: absent
- sporulation efficiency: decreased
- starvation resistance: decreased
- vegetative growth: decreased
- viable
- repressible
- reduction of function
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast GPA1 is homologous to human GNAS and has been used to study mutations found in patients with McCune Albright syndrome
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Gpa1p interacts physically with proteins involved in response to environment; GPA1 interacts genetically with genes involved in chemical response
121 total interactions for 56 unique genes
Physical Interactions
- Affinity Capture-MS: 12
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 35
- Biochemical Activity: 6
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 1
- FRET: 3
- PCA: 2
- Reconstituted Complex: 7
- Two-hybrid: 12
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 7
- Negative Genetic: 3
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 5
- Synthetic Growth Defect: 2
- Synthetic Lethality: 3
- Synthetic Rescue: 14
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 19
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-05-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.