PRP8 / YHR165C Overview
- Standard Name
- PRP8 1
- Systematic Name
- YHR165C
- SGD ID
- SGD:S000001208
- Aliases
- USA2 8 , DBF3 9 10 , DNA39 10 11 , RNA8 12 , SLT21 13
- Feature Type
- ORF , Verified
- Description
- Component of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome; mouse ortholog interacts with androgen receptor and may have a role in prostate cancer 2 3 4 5 6 7
- Name Description
- Pre-mRNA Processing 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- PRP8 is located on the right arm of chromosome VIII between DNA2 flap endonuclease and CDC23 Anaphase-Promoting Complex subunit; dubious ORF YHR165W-A overlaps 5' end of PRP8 on opposite strand; coding sequence is 7242 nucleotides long with 54 SNPs, 14 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Prp8p is 2413 amino acids long, shorter-lived, low in abundance; contains 2 snRNA-binding domains; phosphorylated on 5 residues
- Length (a.a.)
- 2413
- Mol. Weight (Da)
- 279499.3
- Isoelectric Point
- 7.31
- Median Abundance (molecules/cell)
- 1905 +/- 1335
- Half-life (hr)
- 6.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PRP8 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Pre-mRNA intron-binding component of the U4/U6 x U5 tri-snRNP complex; involved in mRNA 3'- and 5'-splice site recognition and conformational rearrangement of the spliceosomal complex containing RNA products from the 1st step of splicing to form catalytic site for the second step of splicing
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (HDA)
- enables pre-mRNA intronic binding (IDA)
- contributes to second spliceosomal transesterification activity (IMP)
- enables U1 snRNA binding (IDA)
- enables U2 snRNA binding (IDA)
- enables U5 snRNA binding (IDA)
- enables U6 snRNA binding (IDA)
Biological Process
- Manually Curated
- involved in generation of catalytic spliceosome for second transesterification step (IMP)
- involved in mRNA 3'-splice site recognition (IMP)
- involved in mRNA 5'-splice site recognition (IMP)
- involved in spliceosomal tri-snRNP complex assembly (IDA, IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; heterozygous null haploinsufficiency results in chromosome instability; reduced function and conditional alleles show increased heat sensitivity, cold sensitivity, broad splicing defect
Classical Genetics
- conditional
- null
- reduction of function
- null
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast PRP8 is homologous to human PRPF8, and has been used to study prostate cancer, myelodysplastic syndrome, and acute myeloid leukemia
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Prp8p interacts physically with proteins involved in mRNA processing; PRP8 interacts genetically with genes involved in mRNA processing
536 total interactions for 257 unique genes
Physical Interactions
- Affinity Capture-MS: 238
- Affinity Capture-RNA: 12
- Affinity Capture-Western: 29
- Co-crystal Structure: 7
- Co-fractionation: 6
- Co-localization: 5
- Co-purification: 3
- Protein-peptide: 2
- Protein-RNA: 5
- Reconstituted Complex: 14
- Two-hybrid: 25
Genetic Interactions
- Dosage Lethality: 3
- Dosage Rescue: 2
- Negative Genetic: 106
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 2
- Positive Genetic: 20
- Synthetic Growth Defect: 14
- Synthetic Lethality: 17
- Synthetic Rescue: 21
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-06-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.