MPT5 / YGL178W Overview

Standard Name
MPT5 1
Systematic Name
HTR1 , UTH4 , PUF5 12
Feature Type
ORF , Verified
mRNA-binding protein of the PUF family; binds to specific mRNAs, often in the 3' UTR; has broad specificity and binds to more than 1000 mRNAs (16% of the transcriptome); recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity 1 2 3 4 5 7 8 9 10 11
Name Description
Multicopy suppressor of Pop Two 6
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

MPT5 is located on the left arm of chromosome VII between TOS3 protein kinase and uncharacterized gene YGL176C; coding sequence is 3220 nucleotides long with an intron at 4..643; of the 11 SNPs, 2 lead to amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Mpt5p is 859 amino acids long, very short-lived, and low in abundance; contians 8 Pumilio RNA-binding repeats; phosphorylated on 29 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
2316 +/- 859
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all MPT5 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Messenger RNA (mRNA) binding involved in glutamate biosynthesis and methionine regeneration; localizes to cytoplasm

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null has elongated cells, decreased lifespan, increased heat sensitivity in W303, increased innate thermotolerance in S288C
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Mpt5p interacts physically with proteins involved in transcription; MPT5 interacts genetically with genes involved in mitotic cell cycle

2305 total interactions for 1854 unique genes

Physical Interactions

  • Affinity Capture-MS: 24
  • Affinity Capture-RNA: 2105
  • Affinity Capture-Western: 8
  • Co-purification: 1
  • PCA: 10
  • Protein-RNA: 3
  • Two-hybrid: 10

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Rescue: 16
  • Negative Genetic: 71
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 5
  • Positive Genetic: 8
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 7
  • Synthetic Rescue: 11
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

MPT5 promoter is bound by Swi4p and Swi6p; MPT5 transcription is upregulated by Ste12p and Tec1p during signaling; Mpt5p downregulates transcription of IXR1 during cell cycle
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.