SPT16 / YGL207W Overview

Standard Name
SPT16 1
Systematic Name
SSF1 , CDC68 20
Feature Type
ORF , Verified
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specifically required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization 2 3 4 5 6 7 8
Name Description
SuPpressor of Ty 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

SPT16 is located on the left arm of chromosome VII toward the telomere betwen SIP2 and CHC1 clathrin heavy chain; coding sequence is 3108 nucleotides long with 25 SNPs, 7 of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Spt16p is 1035 amino acids long, long-lived, moderate-to-high in abundance; ubiquitinylated on K583 and K608, phosphorylated on 17 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
14767 +/- 3107
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

SPT16-AID | spt16-1 | spt16-1 | spt16-11 | spt16-121 | spt16-197 | spt16-A417T,G568S,R569K,P599L | ... Show all

View all SPT16 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Subunit of the FACT, the primosome and the replication fork protection complexes; binds to histones and nucleosomes; involved in regulation of transcription, DNA replication and DNA replication independent nucleosome organization

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; conditional (ts) mutations result in cell cycle arrest at G1 phase; depletion causes chromosome rearrangements and loss; haploinsufficient
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Spt16p interacts physically with proteins involved in transcription; SPT16 interacts genetically with genes involved in transcription

984 total interactions for 518 unique genes

Physical Interactions

  • Affinity Capture-Luminescence: 2
  • Affinity Capture-MS: 547
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 101
  • Biochemical Activity: 18
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 3
  • PCA: 5
  • Reconstituted Complex: 37
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 9
  • Dosage Rescue: 17
  • Negative Genetic: 79
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 11
  • Positive Genetic: 7
  • Synthetic Growth Defect: 58
  • Synthetic Lethality: 30
  • Synthetic Rescue: 29
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

SPT16 promoter is bound by Gcn5p and Yap6p in response to heat; SPT16 transcription is regulated by Sfp1p in response to stress
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2010-03-05

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.