SNF7 / YLR025W Overview
- Standard Name
- SNF7 1
- Systematic Name
- YLR025W
- SGD ID
- SGD:S000004015
- Aliases
- RNS4 21 , VPL5 22 , DID1 11 , VPS32 23 24
- Feature Type
- ORF , Verified
- Description
- One of four core subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; subunit of the endosomal sorting complex required for transport III (ESCRT-III) that forms flat spirals on lipid monolayers as part of the complexes role in driving membrane abscission 2 3 4 6
- Name Description
- Sucrose NonFermenting 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Snf7p is 240 amino acids long, of average half-life, low in abundance; contains disordered region in C-terminus; sumoylated on K79, ubiquitinylated on 3 lysines, phosphorylated on 13 residues
- Length (a.a.)
- 240
- Mol. Weight (Da)
- 26968.4
- Isoelectric Point
- 4.37
- Median Abundance (molecules/cell)
- 7483 +/- 2438
- Half-life (hr)
- 10.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SNF7 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the ESCRT III complex; involved in ubiquitin-dependent protein catabolism via the multivesicular body sorting pathway, late endosome to vacuole transport, intralumenal vesicle formation, ATP export, and the cellular response to anoxia; also localizes to the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein binding (IPI)
Biological Process
- Manually Curated
- involved in ATP export (IMP)
- involved in ESCRT III complex assembly (IDA)
- involved in intralumenal vesicle formation (IMP)
- involved in late endosome to vacuole transport (IMP)
- involved in reticulophagy (IDA)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- part of ESCRT III complex (IDA)
- located in plasma membrane (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- SNF7/YLR025W is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including decreased acid pH resistance, increased biofilm formation, abnormal chemical compound accumulation, increased chemical compound excretion, decreased chronological lifespan, abnormal colony appearance, decreased competitive fitness, decreased desiccation resistance, decreased endocytosis, increased heat sensitivity, decreased hyperosmotic stress resistance, altered metal resistance, increased nutrient utilization, decreased oxidative stress resistance, decreased replicative lifespan, altered resistance to chemicals, decreased respiratory growth rate, decreased sporulation efficiency, decreased telomere length, decreased toxin resistance, decreased transposable element transposition, decreased utilization rates of carbon and nitrogen sources, abnormal vacuolar morphology, decreased vegetative growth rate, decreased acquired thermotolerance, decreased protein secretion, increased protein/peptide accumulation, abnormal protein/peptide distribution and modification, decreased RNA accumulation, and absent vacuolar transport. Reduction of function mutants exhibit abnormal vacuolar transport, while activation mutants show abnormal lipid particle morphology. Overexpression of SNF7 results in decreased vegetative growth, abnormal actin cytoskeleton morphology, increased protein/peptide accumulation, and increased replicative lifespan.
Classical Genetics
- null
- acquired thermotolerance: decreased
- biofilm formation: decreased
- chemical compound accumulation: increased
- heat sensitivity: increased
- metal resistance: decreased
- metal resistance: increased
- protein secretion: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: abnormal
- protein/peptide modification: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- sporulation efficiency: decreased
- sporulation: absent
- telomere length: decreased
- utilization of carbon source: absent
- utilization of carbon source: decreased
- vacuolar morphology: abnormal
- vacuolar transport: absent
- viable
- overexpression
- reduction of function
- unspecified
- null
- acid pH resistance: decreased
- biofilm formation: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- nutrient utilization: increased
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- sporulation: decreased
- telomere length: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- activation
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Snf7p interacts physically with proteins involved in transcription; SNF7 interacts genetically with genes involved in transcription
315 total interactions for 172 unique genes
Physical Interactions
- Affinity Capture-MS: 45
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 48
- Co-fractionation: 1
- Co-localization: 4
- FRET: 1
- PCA: 21
- Reconstituted Complex: 19
- Two-hybrid: 76
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Rescue: 8
- Negative Genetic: 8
- Phenotypic Enhancement: 12
- Phenotypic Suppression: 15
- Synthetic Growth Defect: 24
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 10
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-03-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.