VRP1 / YLR337C Overview
- Standard Name
- VRP1 1
- Systematic Name
- YLR337C
- SGD ID
- SGD:S000004329
- Aliases
- END5 , MDP2 6 , YLR337W
- Feature Type
- ORF , Verified
- Description
- Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) 1 2 3 4 5
- Name Description
- VeRProlin 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 817
- Mol. Weight (Da)
- 82617.7
- Isoelectric Point
- 10.65
- Median Abundance (molecules/cell)
- 3203 +/- 937
- Half-life (hr)
- 8.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all VRP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Actin-binding protein that activates actin nucleation mediated by the Arp2/Arp3 complex; required for bipolar bud site selection, endocytosis and cytokinesis; localizes to the incipient bud site, the bud neck and to actin cortical patches
View computational annotations
Molecular Function
- Manually Curated
- enables actin binding (IPI)
Biological Process
- Manually Curated
- involved in actin cortical patch localization (IMP)
- involved in bipolar cellular bud site selection (IMP)
- involved in endocytosis (IMP)
- involved in positive regulation of Arp2/3 complex-mediated actin nucleation (IDA)
- involved in regulation of cytokinesis (IMP)
Cellular Component
- Manually Curated
- located in actin cortical patch (IDA)
- located in cellular bud neck (IDA)
- located in incipient cellular bud site (IDA)
- located in mating projection tip (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- VRP1/YLR337C is a non-essential gene; null mutants are viable but exhibit a range of phenotypes including decreased bipolar budding pattern, abnormal cell shape, increased chitin deposition, decreased endocytosis, abnormal filamentous growth, decreased growth rate in the exponential phase, decreased hyperosmotic stress resistance, increased metal resistance, abnormal vacuolar morphology, increased cell size, decreased chronological lifespan, abnormal colony appearance, decreased competitive fitness, decreased desiccation resistance, decreased fermentative growth rate, increased heat sensitivity, increased killer toxin resistance, decreased mitotic recombination, decreased oxidative stress resistance, absent respiratory growth, abnormal sporulation, decreased stress resistance, decreased toxin resistance, decreased utilization of carbon source, and decreased vegetative growth rate. Conditional mutants show decreased endocytosis and increased heat sensitivity. Overexpression of VRP1 results in increased invasive growth and decreased vegetative growth rate.
Classical Genetics
- conditional
- null
- bipolar budding pattern: decreased
- cell shape: abnormal
- chemical compound accumulation: increased
- chitin deposition: increased
- endocytosis: decreased
- filamentous growth: abnormal
- growth in exponential phase: decreased rate
- hyperosmotic stress resistance: decreased
- metal resistance: increased
- protein secretion: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- vacuolar morphology: abnormal
- null
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chitin deposition: increased
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: increased
- metal resistance: decreased
- metal resistance: increased
- mitotic recombination: decreased
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- sporulation: abnormal
- stress resistance: decreased
- toxin resistance: decreased
- utilization of carbon source: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
942 total interactions for 665 unique genes
Physical Interactions
- Affinity Capture-MS: 33
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 7
- Co-localization: 3
- Far Western: 1
- FRET: 1
- PCA: 16
- Protein-RNA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 6
- Two-hybrid: 33
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 9
- Negative Genetic: 551
- Phenotypic Enhancement: 6
- Phenotypic Suppression: 5
- Positive Genetic: 198
- Synthetic Growth Defect: 27
- Synthetic Lethality: 30
- Synthetic Rescue: 8
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.