ORM2 / YLR350W Overview
- Standard Name
- ORM2 1
- Systematic Name
- YLR350W
- SGD ID
- SGD:S000004342
- Feature Type
- ORF , Verified
- Description
- Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication 1 2 3 4 5
- Paralog
- ORM1 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- ORM2/YLR350W is located on the right arm of chromosome XII between DIC1 mitochondrial dicarboxylate carrier and NIT3 Nit protein; coding sequence is 651 nucleotides long with 8 SNPs, two of which cause amimo acid polymorphisms; ORM2 has a paralog, ORM1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Orm2p is 216 amino acids long, short-lived, and low in abundance; Orm2p contains a disordered region in its N-terminus, is ubiquitinylated on K25 and K33, and phosphorylated on 13 residues; protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 216
- Mol. Weight (Da)
- 24855.9
- Isoelectric Point
- 8.8
- Median Abundance (molecules/cell)
- 3593 +/- 918
- Half-life (hr)
- 3.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ORM2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- SPOTS complex subunit involved in sphingolipid homeostasis; contributes to negative regulation of sphingolipid biosynthesis and response to unfolded protein; localizes to endoplasmic reticulum and is putatively integral to membrane in high-throughput studies
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in negative regulation of sphingolipid biosynthetic process (IGI, IMP)
- involved in response to unfolded protein (IMP)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (IDA, HDA)
- part of serine palmitoyltransferase complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows increased shingolipid levels; null mutation also increases resistance to bleomycin, camptothecin, and causes abnormal polyphosphate accumulation; overexpression leads to reduced production of sphingoid long-chain bases, ceramides and mature sphingolipids; homozygous null mutation in diploid increases sensitivity to doxorubicin, fenpropimorph, cicloheximide and paromomycin
Classical Genetics
- null
- overexpression
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- metal resistance: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast ORM2 is homologous to human ORMDL3 and ORMDL1, and has been used to study asthma and Loeys-Dietz syndrome
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Orm2p interacts physically with proteins involved in lipid metabolism; ORM2 interacts genetically with genes involved in transcription; the orm2 null mutant is viable, the null mutant of paralog orm1 is viable, the orm2 orm1 double mutant displays a synthetic growth defect
1013 total interactions for 558 unique genes
Physical Interactions
- Affinity Capture-MS: 26
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 24
- Biochemical Activity: 9
- Co-purification: 3
- PCA: 36
- Proximity Label-MS: 1
- Two-hybrid: 4
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 4
- Negative Genetic: 721
- Phenotypic Enhancement: 20
- Phenotypic Suppression: 24
- Positive Genetic: 83
- Synthetic Growth Defect: 26
- Synthetic Lethality: 8
- Synthetic Rescue: 20
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ORM2 promoter is bound by Spt3p, Xbp1p, and Yap6p in response to heat; ORM2 transcription is regulated by Reb1p; Orm2p protein stability is downregulated by TUL1 E3 ubiquitin ligase complex; Orm2 protein activity is regulated by Hog1p in response to osmotic stress, and also by Swe1p
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-08-19
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.