Protein Help

NDI1 / YML120C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Protein Product
NADH-ubiquinone reductase (H(+)-translocating) NDI1
Feature Type
ORF , Verified
EC Number
1.6.5.9
Summary
Ndi1p is 513 amino acids long, shorter-lived, low in abundance; contains 4 FAD-dependent pyridine nucleotide reductase signature domains; phosphorylated on T28, succinylated on 18 lysines

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life8.4 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
7596SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
6931SD0.2 M hydroxyurea160 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
8178SD200 ng/ml sirolimus700 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
5543YEPDuntreatedflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
12763YEPDcell quiescence7 dflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
Showing 1 to 5 of 25 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


ndi1-Δ

View all NDI1 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH SDKPNVLILG
61 SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG TVDEKSIIEP IVNFALKKKG
121 NVTYYEAEAT SINPDRNTVT IKSLSAVSQL YQPENHLGLH QAEPAEIKYD YLISAVGAEP
181 NTFGIPGVTD YGHFLKEIPN SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG
241 VEAAGELQDY VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV
301 HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL FKKIPEQNSS
361 KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA EYLAKNFDKM AQIPNFQKNL
421 SSRKDKIDLL FEENNFKPFK YNDLGALAYL GSERAIATIR SGKRTFYTGG GLMTFYLWRI
481 LYLSMILSAR SRLKVFFDWI KLAFFKRDFF KGL*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

19 entries for 19 sites

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SiteModificationModifierReference
T28phosphorylated residueReinders J, et al. (2007) PMID: 17761666
K41succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K76succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K119succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K214succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K220succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K284succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K299succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K308succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
K312succinylated residueFrankovsky J, et al. (2021) PMID: 34480900
Showing 1 to 10 of 19 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A417.99
C00.00
D203.90
E316.04
F285.46
G336.43
H112.14
I346.63
K438.38
L5610.92
M61.17
N295.65
P265.07
Q152.92
R224.29
S367.02
T316.04
V295.65
W40.78
Y183.51

Physical Details

Length (a.a): 513
Molecular Weight (Da): 57264.4
Isoelectric Point (pl): 9.96
Formula: C2606H4126N692O745S6
Aliphatic Index: 89.01
Instability Index: 35.53

Coding Region Translation Calculations

Codon Bias: 0.25
Codon Adaptation Index: 0.19
Frequence of Optimal Codons: 0.57
Hydropathicity of Protein: -0.23
Aromaticity Score: 0.1

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 48820.0
NO Cys residues appear as half cystines: 48820.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

58 entries for 10 sources


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External IDSource
5554DIP
1.6.5.9ExPASy
X61590GenBank/EMBL/DDBJ
AY723851GenBank/EMBL/DDBJ
6323515GenBank/EMBL/DDBJ
CAA43787.1GenBank/EMBL/DDBJ
AAU09768.1GenBank/EMBL/DDBJ
CAA89160.1GenBank/EMBL/DDBJ
Z49218GenBank/EMBL/DDBJ
403072242GenBank/EMBL/DDBJ
Showing 1 to 10 of 58 entries

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