NDI1 / YML120C Overview
- Standard Name
- NDI1 1
- Systematic Name
- YML120C
- SGD ID
- SGD:S000004589
- Feature Type
- ORF , Verified
- Description
- NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in respiratory chain but does not pump protons, in contrast to higher eukaryotic multisubunit respiratory complex I; upon apoptotic stress, is activated in mitochondria by N-terminal cleavage, then translocates to cytoplasm to induce apoptosis; homolog of human AIFM2; yeast NDI1 complements several phenotypes of human cell line with mutated MT-ND4, implicated in Leber hereditary optic neuropathy 1 2 3 4 5 6
- Name Description
- NADH Dehydrogenase Internal 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ndi1p is 513 amino acids long, shorter-lived, low in abundance; contains 4 FAD-dependent pyridine nucleotide reductase signature domains; phosphorylated on T28, succinylated on 18 lysines
- Length (a.a.)
- 513
- Mol. Weight (Da)
- 57264.4
- Isoelectric Point
- 9.96
- Median Abundance (molecules/cell)
- 7696 +/- 2456
- Half-life (hr)
- 8.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NDI1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- NADH:ubiquinone oxidoreductase involved in mitochondrial electron transport and positive regulation of apoptosis; localizes to mitochondrial matrix
View computational annotations
Molecular Function
- Manually Curated
- enables NADH dehydrogenase (ubiquinone) activity (IDA)
Biological Process
- Manually Curated
- involved in mitochondrial electron transport, NADH to ubiquinone (IDA)
- involved in NADH oxidation (IDA)
- involved in positive regulation of apoptotic process (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial matrix (IMP, IDA)
- located in mitochondrion (HDA, IDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation results in decreased apoptosis, replicative lifespan and respiratory growth; overexpression causes increased accumulation of reactive oxygen species
Classical Genetics
- null
- apoptosis: decreased
- chemical compound accumulation: increased
- lifespan: increased
- mitochondrial genome maintenance: abnormal
- replicative lifespan: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- sporulation efficiency: decreased
- toxin resistance: increased
- utilization of carbon source: absent
- utilization of carbon source: decreased
- overexpression
- null
- biofilm formation: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- growth in exponential phase: arrested
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast NDI1 is homologous to human MT-ND4, and has been used to study Leber hereditary optic neuropathy
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Ndi1p interacts physically with proteins involved in mitotic cell cycle; NDI1 interacts genetically with genes involved in lipid metabolism
111 total interactions for 98 unique genes
Physical Interactions
- Affinity Capture-MS: 27
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 1
- Co-crystal Structure: 3
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 4
- PCA: 1
- Proximity Label-MS: 5
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Negative Genetic: 39
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 4
- Positive Genetic: 12
- Synthetic Lethality: 4
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-15
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.