PAH1 / YMR165C Overview


Standard Name
PAH1 1
Systematic Name
YMR165C
SGD ID
SGD:S000004775
Aliases
SMP2 20
Feature Type
ORF , Verified
Description
Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; regulates phospholipid synthesis, nuclear/ER membrane growth, lipid droplet formation, triacylglycerol synthesis, vacuolar homeostasis and cell wall integrity; phosphorylated by Hsl1p, Pho85p/Pho80p, Cdc28p/Cyclin B, PKA, PKC, and CKII, regulating activity, localization, and proteosomal degradation; homolog of mammalian lipins 1 and 2; human homologs LPIN1, LPIN2, LPIN3 complement the null 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
Name Description
Phosphatidic Acid phosphoHydrolase 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PAH1 is located on the right arm of chromosome XIII between MSS11 transcription factor and MME1 mitochondrial magnesium exporter; coding sequence is 2589 nucleotides long with 22 SNPs, 6 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Pah1p is 862 amino acids long, shorter-lived, low in abundance; acetylated on K496 and K801, phosphorylated on 59 residues; Pah1 protein stability is increased via phosphorylation by PHO80-PHO85 kinase complex, Pah1 protein stability is decreased via dephosphorylation by Nem1-Spo7 phosphatase complex; Pah1p activity is inhibited by Rim11p, and activated by Yck1p
Length (a.a.)
862
Mol. Weight (Da)
94989.7
Isoelectric Point
4.6
Median Abundance (molecules/cell)
2607 +/- 879
Half-life (hr)
8.4

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all PAH1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Phosphatidate phosphatase involved in phospholipid biosynthesis, triglyceride biosynthesis, and lipid particle organization; also involved in aerobic respiration, plasmid maintenance, and non-autophagic vacuole fusion; localizes to the vacuole, lipid particle, cytosol, and nuclear membrane

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; in stationary phase, null mutant exhibits increased phospholipid and fatty acid levels and decreased triglyceride levels; null and conditional mutants have abnormal nuclear and ER morphology and lack vacuolar fusion; mutant is hypersensitive to K1 killer toxin; in large-scale studies, null mutant has reduced competitive fitness and is sensitive to various chemicals
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast PAH1 is homologous to human LPIN1, LPIN2, and LPIN3, and has been used to study myoglobinuria and lipid metabolism disorder
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Pah1p interacts physically with proteins involved in mitotic cell cycle; PAH1 interacts genetically with genes involved in lipid metabolism

354 total interactions for 231 unique genes

Physical Interactions

  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 4
  • Biochemical Activity: 59
  • Co-localization: 2
  • PCA: 5
  • Protein-peptide: 1
  • Protein-RNA: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Rescue: 8
  • Negative Genetic: 145
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 16
  • Positive Genetic: 46
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 13
  • Synthetic Rescue: 10
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
PAH1 transcription is downregulated by Spt10p, PAH1 transcription is upregulated by Zap1p in response to zinc ion starvation; Pah1 protein stability is increased via phosphorylation by PHO80-PHO85 kinase complex, Pah1 protein stability is decreased via dephosphorylation by Nem1-Spo7 phosphatase complex; Pah1p activity is inhibited by Rim11p, and activated by Yck1p
Regulators
32
Targets
3
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2024-05-31

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
90
Additional
54
Reviews
78

Resources