PAH1 / YMR165C Overview
- Standard Name
- PAH1 1
- Systematic Name
- YMR165C
- SGD ID
- SGD:S000004775
- Aliases
- SMP2 20
- Feature Type
- ORF , Verified
- Description
- Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; regulates phospholipid synthesis, nuclear/ER membrane growth, lipid droplet formation, triacylglycerol synthesis, vacuolar homeostasis and cell wall integrity; phosphorylated by Hsl1p, Pho85p/Pho80p, Cdc28p/Cyclin B, PKA, PKC, and CKII, regulating activity, localization, and proteosomal degradation; homolog of mammalian lipins 1 and 2; human homologs LPIN1, LPIN2, LPIN3 complement the null 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
- Name Description
- Phosphatidic Acid phosphoHydrolase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pah1p is 862 amino acids long, shorter-lived, low in abundance; acetylated on K496 and K801, phosphorylated on 59 residues; Pah1 protein stability is increased via phosphorylation by PHO80-PHO85 kinase complex, Pah1 protein stability is decreased via dephosphorylation by Nem1-Spo7 phosphatase complex; Pah1p activity is inhibited by Rim11p, and activated by Yck1p
- Length (a.a.)
- 862
- Mol. Weight (Da)
- 94989.7
- Isoelectric Point
- 4.6
- Median Abundance (molecules/cell)
- 2607 +/- 879
- Half-life (hr)
- 8.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PAH1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phosphatidate phosphatase involved in phospholipid biosynthesis, triglyceride biosynthesis, and lipid particle organization; also involved in aerobic respiration, plasmid maintenance, and non-autophagic vacuole fusion; localizes to the vacuole, lipid particle, cytosol, and nuclear membrane
View computational annotations
Molecular Function
- Manually Curated
- enables phosphatidate phosphatase activity (IDA, IMP)
- enables transcription cis-regulatory region binding (IDA)
Biological Process
- Manually Curated
- involved in aerobic respiration (IMP)
- involved in diacylglycerol biosynthetic process (IDA)
- involved in lipid droplet organization (IMP, IGI)
- involved in phospholipid biosynthetic process (IMP)
- involved in plasmid maintenance (IMP)
- involved in triglyceride biosynthetic process (IMP, IGI)
- involved in vacuole fusion, non-autophagic (IMP)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; in stationary phase, null mutant exhibits increased phospholipid and fatty acid levels and decreased triglyceride levels; null and conditional mutants have abnormal nuclear and ER morphology and lack vacuolar fusion; mutant is hypersensitive to K1 killer toxin; in large-scale studies, null mutant has reduced competitive fitness and is sensitive to various chemicals
Classical Genetics
- null
- chemical compound accumulation: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- heat sensitivity: increased
- lipid particle morphology: abnormal
- nuclear morphology: abnormal
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- RNA accumulation: increased
- vacuolar morphology: abnormal
- unspecified
- reduction of function
- conditional
- overexpression
- null
- unspecified
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast PAH1 is homologous to human LPIN1, LPIN2, and LPIN3, and has been used to study myoglobinuria and lipid metabolism disorder
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pah1p interacts physically with proteins involved in mitotic cell cycle; PAH1 interacts genetically with genes involved in lipid metabolism
353 total interactions for 230 unique genes
Physical Interactions
- Affinity Capture-MS: 8
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 4
- Biochemical Activity: 59
- Co-localization: 2
- PCA: 5
- Protein-peptide: 1
- Protein-RNA: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 8
- Negative Genetic: 145
- Phenotypic Enhancement: 11
- Phenotypic Suppression: 16
- Positive Genetic: 46
- Synthetic Growth Defect: 17
- Synthetic Lethality: 13
- Synthetic Rescue: 10
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- PAH1 transcription is downregulated by Spt10p, PAH1 transcription is upregulated by Zap1p in response to zinc ion starvation; Pah1 protein stability is increased via phosphorylation by PHO80-PHO85 kinase complex, Pah1 protein stability is decreased via dephosphorylation by Nem1-Spo7 phosphatase complex; Pah1p activity is inhibited by Rim11p, and activated by Yck1p
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-05-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.