MGM1 / YOR211C Overview
- Standard Name
- MGM1 1
- Systematic Name
- YOR211C
- SGD ID
- SGD:S000005737
- Aliases
- MNA1 12
- Feature Type
- ORF , Verified
- Description
- Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mitochondrial morphology, fusion, and genome maintenance; promotes membrane bending; plays a direct role in formation and maintenance of lamellar, but not of tubular, cristae; exists as long and short form with different distributions; ratio of long to short forms is regulated by Psd1p; homolog of human OPA1 involved in autosomal dominant optic atrophy 2 3 4 5 6 7 8 9 10 11
- Name Description
- Mitochondrial Genome Maintenance 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mgm1p is 881 amino acids long, shorter-lived, low in abundance; contains a disordered region in the N-terminus and 6 dynamin domains; succinylated on K274, phosphorylated on S463, ubiquitinylated on K549
- Length (a.a.)
- 881
- Mol. Weight (Da)
- 99178.8
- Isoelectric Point
- 7.67
- Median Abundance (molecules/cell)
- 4269 +/- 1047
- Half-life (hr)
- 8.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MGM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTPase protein involved in mitochondrial membrane fusion and mitochondrial genome maintenance; localized to the inner mitochondrial membrane, as well as mitochondrial intermembrane space and crista
View computational annotations
Molecular Function
- Manually Curated
- enables cardiolipin binding (IDA)
- enables GTPase activity (IDA, ISA)
- enables phosphatidic acid binding (IDA)
- enables phosphatidylinositol-3,5-bisphosphate binding (IDA)
- enables phosphatidylserine binding (IDA)
Biological Process
- Manually Curated
- involved in cristae formation (IMP)
- involved in heme transport (IMP)
- involved in mitochondrial fusion (IMP)
- involved in mitochondrial genome maintenance (IMP)
- involved in mitochondrion organization (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial crista (IDA)
- is active in mitochondrial inner boundary membrane (IDA)
- located in mitochondrial inner membrane (IDA)
- located in mitochondrial intermembrane space (IDA)
- located in mitochondrial outer membrane (IDA)
- located in mitochondrion (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- MGM1/YOR211C is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including abnormal mitochondrial genome maintenance and morphology, increased replicative lifespan, decreased respiratory growth rate, decreased small molecule transport, increased toxin resistance, increased biofilm formation, decreased chronological lifespan, decreased competitive fitness, decreased endocytosis, increased heat sensitivity, increased metal resistance, decreased starvation resistance, increased stress resistance, decreased utilization rates of carbon and nitrogen sources, decreased UV resistance, abnormal vacuolar morphology, and decreased vegetative growth rate. Reduction of function mutants show abnormal mitochondrial morphology and genome maintenance, and decreased respiratory growth. Conditional mutants display abnormal mitochondrial morphology. Overexpression of MGM1 results in decreased mitochondrial genome maintenance, abnormal mitochondrial morphology, and decreased vegetative growth.
Classical Genetics
- null
- reduction of function
- overexpression
- conditional
- null
- biofilm formation: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- endocytosis: decreased
- heat sensitivity: increased
- metal resistance: increased
- mitochondrial genome maintenance: decreased
- mitochondrial morphology: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- starvation resistance: decreased
- stress resistance: increased
- toxin resistance: increased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MGM1 is homologous to human OPA1, and has been used to study mutations found in patients with mitochondrial encephalopathy and various forms of optic atrophy, Behr syndrome, and multi-system recessive mitochondrial disorders
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mgm1p interacts physically with proteins involved in mitochondrion organization; MGM1 interacts genetically with genes involved in mitotic cell cycle
296 total interactions for 243 unique genes
Physical Interactions
- Affinity Capture-MS: 8
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 7
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Co-fractionation: 2
- PCA: 2
- Reconstituted Complex: 3
- Two-hybrid: 3
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 172
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 3
- Positive Genetic: 72
- Synthetic Growth Defect: 4
- Synthetic Lethality: 3
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MGM1 promoter is bound by Sua7p in response to heat
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-21
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.