TUB1 / YML085C Overview
- Standard Name
- TUB1 1
- Systematic Name
- YML085C
- SGD ID
- SGD:S000004550
- Feature Type
- ORF , Verified
- Description
- Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication 2 3 4
- Name Description
- TUBulin 1
- Paralog
- TUB3 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TUB1 is located on the left arm of chromosome XIII between ALO1 D-arabinono-1,4-lactone oxidase and uncharacterized gene YML083C; coding sequence is 1460 nucleotides long with 6 SNPs and an intron at 26..141 that contains a variable run of T nucleotides; TUB1 has paralog TUB3 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Alpha-tubulin Tub1p is 447 amino acids long, long-lived, and moderate in abundance; contains 13 alpha tubulin domains; phosphorylated on 5 residues, sumoylated on 2 lysines, ubiquitinylated on 1 lysine; associates with beta-tubulin Tub2p to form tubulin dimer; relative distribution to nuclear foci increases upon DNA replication stress
- Length (a.a.)
- 447
- Mol. Weight (Da)
- 49778.4
- Isoelectric Point
- 4.71
- Median Abundance (molecules/cell)
- 11926 +/- 7484
- Half-life (hr)
- 19.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TUB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Tubulin complex subunit; involved in homologous chromosome segregation, mitotic sister chromatid segregation, and microtubule-mediated transport of the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in homologous chromosome segregation (IMP)
- involved in mitotic sister chromatid segregation (IMP)
- involved in nuclear migration along microtubule (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- located in nuclear periphery (IDA)
- located in nucleus (HDA)
- part of tubulin complex (IDA, IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; heterozygous diploid null mutants are haploinsufficient and sensitive to heat, TOR inhibitor rapamycin, and actin polymerization inhibitor latrunculin A; conditional mutants have abnormal spindles at cold temperatures; overexpression interferes with the cell cycle and slows growth
Classical Genetics
- overexpression
- conditional
- null
- reduction of function
- unspecified
- null
- overexpression
- repressible
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast TUB1 is homologous to human TUBA1A and TUBA4A, and has been used to study tubulin mutations in brain development disorders, macrothrombocytopenia, and amyotrophic lateral sclerosis type 22
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Tub1p interacts physically with proteins involved in mitosis; TUB1 interacts genetically with genes involved in mitosis; the tub1 null mutant is inviable; the null mutant of paralog tub3 is viable; the tub1 tub3 double mutant is inviable.
490 total interactions for 385 unique genes
Physical Interactions
- Affinity Capture-MS: 133
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 14
- Co-crystal Structure: 9
- Co-fractionation: 2
- Co-localization: 11
- Co-purification: 4
- Proximity Label-MS: 2
- Reconstituted Complex: 27
- Two-hybrid: 17
Genetic Interactions
- Dosage Lethality: 3
- Dosage Rescue: 18
- Negative Genetic: 93
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 7
- Positive Genetic: 96
- Synthetic Growth Defect: 25
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 15
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2003-12-18
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.