SPT20 / YOL148C Overview
- Standard Name
- SPT20 1
- Systematic Name
- YOL148C
- SGD ID
- SGD:S000005508
- Aliases
- ADA5 4
- Feature Type
- ORF , Verified
- Description
- Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay 2 3
- Name Description
- SuPpressor of Ty 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 604
- Mol. Weight (Da)
- 67816.7
- Isoelectric Point
- 10.14
- Median Abundance (molecules/cell)
- 2108 +/- 896
- Half-life (hr)
- 9.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPT20 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcriptional coregulator protein that is a subunit of the SAGA and SLIK complexes; involved in chromatin organization, histone acetylation and IRE1-mediated unfolded protein response
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coregulator activity (IMP)
Biological Process
- Manually Curated
- involved in chromatin organization (IDA)
- involved in IRE1-mediated unfolded protein response (IMP)
Cellular Component
- Manually Curated
- part of SAGA complex (IDA)
- part of SLIK (SAGA-like) complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- cold sensitivity: increased
- cytoskeleton morphology: abnormal
- heat sensitivity: increased
- metal resistance: decreased
- metal resistance: increased
- osmotic stress resistance: decreased
- oxidative stress resistance: decreased
- protein/peptide modification: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- vegetative growth: decreased
- X ray resistance: increased
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- bud morphology: abnormal
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- freeze-thaw resistance: decreased
- haploinsufficient
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- metal resistance: increased
- mitophagy: increased
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- sporulation: absent
- stress resistance: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased
- vegetative growth: absent
- vegetative growth: decreased rate
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
404 total interactions for 104 unique genes
Physical Interactions
- Affinity Capture-MS: 217
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 47
- Biochemical Activity: 1
- Co-fractionation: 3
- Co-localization: 5
- Co-purification: 39
- Reconstituted Complex: 8
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 1
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 2
- Synthetic Growth Defect: 14
- Synthetic Lethality: 39
- Synthetic Rescue: 13
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SPT20 (also known as ADA5) encodes a subunit of SAGA, a histone acetyltransferase and transcriptional coactivator complex that regulates transcription of a subset of RNA polymerase II-dependent genes. Spt20p is also found in an altered SAGA complex, termed SLIK (SAGA-like) or SALSA (SAGA altered, Spt8 absent) and is involved in maintaining the integrity of these complexes as intact SAGA complexes cannot be purified in the absence of Spt20p. Spt20p and SAGA has also been shown to play a role in the ER unfolded protein response (UPR) controlling the splicing of HAC1 mRNA, the major transcription factor of the UPR.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.