VMA21 / YGR105W Overview
- Standard Name
- VMA21 1
- Systematic Name
- YGR105W
- SGD ID
- SGD:S000003337
- Feature Type
- ORF , Verified
- Description
- Integral membrane protein required for V-ATPase function; not actual component of vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human VMA21/XMEA (X-linked Myopathy with Excessive Autophagy); functions in assembly of V-ATPase; localizes to endoplasmic reticulum (ER) 3 4 5 6 7
- Name Description
- Vacuolar Membrane Atpase 2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Vma21p is 77 amino acids long, low in abundance
- Length (a.a.)
- 77
- Mol. Weight (Da)
- 8433.1
- Isoelectric Point
- 4.84
- Median Abundance (molecules/cell)
- 3010 +/- 1238
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all VMA21 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Endoplasmic reticulum membrane protein involved in assembly of the vacuolar proton-transporting V-type ATPase complex
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA)
- located in endoplasmic reticulum membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- VMA21/YGR105W is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including delayed fermentative metabolism, decreased gamma ray resistance, decreased metal resistance, decreased oxidative stress resistance, decreased replicative lifespan, altered resistance to chemicals, absent or decreased respiratory growth rate, decreased alkaline pH resistance, auxotrophy, decreased chronological lifespan, decreased competitive fitness, decreased desiccation resistance, decreased freeze-thaw resistance, decreased hyperosmotic stress resistance, decreased innate thermotolerance, decreased mitotic recombination, increased mutation frequency, decreased resistance to enzymatic treatment, decreased toxin resistance, decreased utilization of carbon and iron sources, abnormal vacuolar morphology, and decreased vegetative growth rate. Reduction of function mutants show decreased acid and alkaline pH resistance. Overexpression of VMA21 results in increased invasive growth.
Classical Genetics
- null
- chemical compound accumulation: increased
- fermentative metabolism: delayed
- gamma ray resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- reduction of function
- null
- alkaline pH resistance: decreased
- auxotrophy
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- freeze-thaw resistance: decreased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- metal resistance: decreased
- mitotic recombination: decreased
- mutation frequency: increased
- oxidative stress resistance: decreased
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased rate
- respiratory growth: increased
- toxin resistance: decreased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of iron source: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast VMA21 is homologous to human VMA21, and has been used to study follicular lymphoma and X-linked myopathy with excessive autophagy (XMEA)
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Vma21p interacts physically with proteins involved in intracellular ion homeostasis; VMA21 interacts genetically with genes involved in transcription
1109 total interactions for 923 unique genes
Physical Interactions
- Affinity Capture-MS: 19
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 6
- Co-purification: 1
- PCA: 12
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 433
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 1
- Positive Genetic: 112
- Synthetic Growth Defect: 509
- Synthetic Lethality: 5
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-21
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.