NDD1 / YOR372C Overview
- Standard Name
- NDD1 1
- Systematic Name
- YOR372C
- SGD ID
- SGD:S000005899
- Feature Type
- ORF , Verified
- Description
- Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes; turnover is tightly regulated during cell cycle and in response to DNA damage 1 2
- Name Description
- Nuclear Division Defective 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 554
- Mol. Weight (Da)
- 60537.5
- Isoelectric Point
- 10.1
- Median Abundance (molecules/cell)
- 511 +/- 451
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NDD1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcriptional coactivator involved in the G2/M transition of the mitotic cell cycle; found in the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coactivator activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in G2/M transition of mitotic cell cycle (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- repressible
- overexpression
- unspecified
- null
- overexpression
- repressible
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
51 total interactions for 31 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 7
- Biochemical Activity: 9
- Co-localization: 1
- Co-purification: 1
- PCA: 3
- Protein-RNA: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 5
- Dosage Rescue: 2
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Synthetic Growth Defect: 2
- Synthetic Lethality: 1
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- NDD1 encodes a transcriptional coactivator that regulates progression of the cell cycle in concert with the transcription factors Mcm1p and Fkh2p. Its targets are genes expressed in the G2/M phase of the cell cycle, including cyclin genes CLB1 and CLB2, the CDC5 polo-like kinase gene, the CDC20 component of the APC ubiquitin ligase, and the transcription factor genes ACE2 and SWI5. Fkh2p and Mcm1p bind together at target promoters and repress transcription in the absence of Ndd1p. NDD1 transcription is activated during S phase by the Hcm1p transcription factor, and once Ndd1p is expressed, it binds to Fkh2p at target promoters. This binding is enhanced by phosphorylation by the Cdc28p/Clb2p and Cdc5p kinases, and the binding of phosphorylated Ndd1p to Fkh2p causes transcriptional activation of target genes. If DNA damage occurs, the Rad53p and Dun1p kinases phosphorylate Ndd1p to downregulate it, preventing its binding to Fkh2p at promoters and thus arresting the cell cycle. At the end of M phase, Ndd1p is targeted for degradation by the APC-Cdh1p ubiquitin ligase.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.