Although the human ULK complex mediates phagophore initiation similar to the budding yeast Saccharomyces cerevisiae ATG1 complex, this complex contains ATG101 but not ATG29 and ATG31. Here, we analyzed the fission yeast Schizosaccharomyces pombe ATG1 complex, which has a subunit composition that resembles the human ULK complex. Our pairwise coprecipitation experiments showed that while the interactions between ATG1, ATG13, and ATG17 are conserved, ATG101 does not bind ATG17. Instead, ATG101 interacts with the HORMA domain of ATG13 and this enhances the stability of both proteins. We also found that S. pombe ATG17, the putative scaffold subunit, adopts a rod-shaped structure with no discernible curvature. Interestingly, S. pombe ATG17 binds S. cerevisiae ATG13, ATG29, and ATG31 in vitro, but it cannot complement the function of S. cerevisiae ATG17 in vivo. Furthermore, S. pombe ATG101 cannot substitute for the function of S. cerevisiae ATG29 and ATG31 in vivo. Collectively, our work generates new insights into the subunit organization and structural properties of an ATG101-containing ATG1/ULK complex.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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