Background: Saccharomyces cerevisiae is susceptible to high-sugar stress in the production of bioethanol, wine and bread. Calcium signal is widely involved in various physiological and metabolic activities of cells. The present study aimed to explore the effects of Ca²⁺ signal on the antioxidant mechanism of yeast during high-sugar fermentation.

Results: Compared to yeast without available Ca²⁺, yeast in the high glucose with Ca²⁺ group had higher dry weight, higher ethanol output at 12 and 24 h and higher glycerol output at 24 and 36 h. During the whole growth process, the trehalose synthesis capacity of yeast in the high glucose with Ca²⁺ group was lower and intracellular reactive oxygen species content was higher compared to yeast without available Ca²⁺. Intracellular malondialdehyde content of yeast under high glucose with Ca²⁺ was significantly lower than yeast under high glucose without available Ca²⁺ except for 6 h. The superoxide dismutase and catalase activities of yeast and glutathione content were higher in the high glucose with Ca²⁺ group compared to yeast in high glucose without available Ca²⁺. The expression levels of SOD1, GSH1, GPX2 genes were higher for high glucose without available Ca²⁺ at 6 h, while yeast in the high glucose with Ca²⁺ group had a higher expression of antioxidant-related genes except SOD1 and CTT1 at 12 h. The expression levels of antioxidant-related genes of yeast for high glucose with Ca²⁺ were higher at 24 h, and those of genes except SOD1 of yeast in the high glucose with Ca²⁺ group were higher at 36 h.

Conclusion: High-glucose stress limited the growth of yeast, while a moderate extracellular Ca²⁺ signal could improve the antioxidant capacity of yeast in a high-glucose environment by regulating protectant metabolism and enhancing the antioxidant enzyme activity and expression of antioxidant genes in a high-sugar environment. © 2024 Society of Chemical Industry.

• PMID: 38390983
• DOI full text
• PubMed
• PubTator

Reference Type

Journal Article

Authors

Xie D, Sun Y, Li X, Zheng J, Ren S

Primary Lit For

Additional Lit For

Review For