GPD1 / YDL022W Overview
- Standard Name
- GPD1 1 2
- Systematic Name
- YDL022W
- SGD ID
- SGD:S000002180
- Aliases
- DAR1 8 , HOR1 9 , OSG1 10 , OSR5
- Feature Type
- ORF , Verified
- Description
- NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import 3 4 5 6 7
- Name Description
- Glycerol-3-Phosphate Dehydrogenase 3
- Paralog
- GPD2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Gpd1p is 391 amino acids long, very long-lived, high in abundance; acetylated on 3 lysines, phosphorylated on 10 residues; protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 391
- Mol. Weight (Da)
- 42854.2
- Isoelectric Point
- 5.16
- Median Abundance (molecules/cell)
- 26223 +/- 15467
- Half-life (hr)
- 24.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GPD1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- NAD-dependent glycerol-3-phosphate dehydrogenase with key role in synthesis of glycerol and in intracellular glycerol accumulation in response to high-osmolarity stress; localized to the cytoplasm and peroxisomes
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in intracellular accumulation of glycerol (IMP)
- involved in NADH oxidation (IMP)
- involved in protein import into peroxisome matrix (IMP)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- located in mitochondrion (HDA)
- located in nucleus (IDA)
- located in peroxisome (IDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows increased accumulation of ethanol and decreased accumulation of glycerol, also shows sensitivity to hyperosmotic stress; overexpression causes increased accumulation of glycerol, acetate, acetoin and butane-2,3-diol
Classical Genetics
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- hyperosmotic stress resistance: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vegetative growth: decreased rate
- overexpression
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Gpd1p interacts physically with proteins involved in transcription and RNA catabolism; GPD1 interacts genetically with genes involved in lipid metabolism; the gpd1 null mutant is viable, the null mutant of paralog gpd2 is viable, the gpd1 gpd2 double mutant is inviable or displays a growth defect
193 total interactions for 158 unique genes
Physical Interactions
- Affinity Capture-MS: 34
- Affinity Capture-RNA: 51
- Affinity Capture-Western: 2
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 1
- Proximity Label-MS: 2
- Two-hybrid: 3
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 13
- Negative Genetic: 48
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 3
- Positive Genetic: 5
- Synthetic Growth Defect: 11
- Synthetic Lethality: 3
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- GPD1 promoter is bound by several transcription factors in response to heat, including Leu3p, Med2p, Msn2p, Rgr1p, Spt3p, Spt6p, Sua7p, and Yap1p; GPD1 transcription is regulated by several transcription factors including Abf1p, Aft1p, Cad1p, Cst6p, Gcr1p, Hfi1p, Rap1p, Rim101p, Sin4p, and Spt20p
- Regulators
- 22
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-08
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.