SSC1 / YJR045C Overview
- Standard Name
- SSC1 1
- Systematic Name
- YJR045C
- SGD ID
- SGD:S000003806
- Aliases
- mtHSP70 , ENS1 2
- Feature Type
- ORF , Verified
- Description
- Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication 2 3 4 5 6
- Name Description
- Stress-Seventy subfamily C
- Paralog
- ECM10 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SSC1/YFR045C is located on the right arm of chromosome X between VPS35 late endosomal protein and TAH11DNA replication licensing factor; coding sequence is 1965 nucleotides long with 11 SNPs, 3 of which cause amino acid polymorphisms, and an in-frame 3-nucleotide deletion in strains D273-10B, Sigma1278b, and RM11-1a that removes Asn643; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ssc1p is 654 amino acids long, longer-lived, moderate in abundance; contains 9 70kDa heat shock protein signature domains and a disordered region at the C-terminus; acetylated on Met1 and 18 lysines, succinylated on 35 lyines, phosphorylated on 18 residues
- Length (a.a.)
- 654
- Mol. Weight (Da)
- 70620.5
- Isoelectric Point
- 5.24
- Median Abundance (molecules/cell)
- 54905 +/- 10911
- Half-life (hr)
- 12.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SSC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATPase involved in protein import into mitochondrial matrix and protein refolding and unfolding
View computational annotations
Molecular Function
- Manually Curated
- enables ATP hydrolysis activity (IDA)
- enables enzyme regulator activity (IDA)
Biological Process
- Manually Curated
- involved in protein import into mitochondrial matrix (IDA, IMP)
- involved in protein refolding (IDA, IMP)
- involved in protein unfolding (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial inner membrane (IDA)
- located in mitochondrial nucleoid (IDA)
- located in mitochondrion (HDA)
- part of PAM complex, Tim23 associated import motor (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- SSC1/YJR045C is a non-essential gene; null mutants are inviable but exhibit increased chronological and replicative lifespan, increased protein/peptide accumulation, and decreased respiratory growth rate. Reduction of function mutants show increased chemical compound accumulation, abnormal mitochondrial morphology, decreased mitochondrial transport, decreased oxidative stress resistance, decreased protein activity, decreased respiratory growth, decreased vegetative growth, and decreased competitive fitness. Overexpression of SSC1 results in increased heat sensitivity, abnormal protein/peptide distribution, absent respiratory growth, decreased vegetative growth, and increased invasive growth. Conditional mutants display increased chemical compound accumulation, increased cold and heat sensitivity, abnormal mitochondrial genome maintenance and morphology, decreased mitochondrial transport, decreased oxidative stress resistance, decreased protein activity, increased protein/peptide accumulation, absent or decreased respiratory growth, decreased vegetative growth, decreased resistance to chemicals, increased toxin resistance, and decreased size of the nucleolus.
Classical Genetics
- null
- reduction of function
- overexpression
- conditional
- chemical compound accumulation: increased
- cold sensitivity: increased
- heat sensitivity: increased
- mitochondrial genome maintenance: abnormal
- mitochondrial morphology: abnormal
- mitochondrial transport: decreased
- oxidative stress resistance: decreased
- protein activity: decreased
- protein/peptide accumulation: increased
- respiratory growth: absent
- respiratory growth: decreased
- vegetative growth: decreased
- null
- reduction of function
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SSC1 is homologous to human HSPA9, and has been used to study Parkinson's disease
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Ssc1p interacts physically with proteins invovled in mitochondrial translation; SSC1 interacts genetically with genes involved in transcription; the ssc1 null mutant is inviable, the null mutant of paralog ecm10 is viable, the ssc1 ecm10 double mutant displays a synthetic growth defect
1364 total interactions for 1000 unique genes
Physical Interactions
- Affinity Capture-MS: 682
- Affinity Capture-RNA: 14
- Affinity Capture-Western: 81
- Biochemical Activity: 4
- Co-fractionation: 1
- Co-purification: 7
- Cross-Linking-MS (XL-MS): 19
- FRET: 1
- PCA: 1
- Proximity Label-MS: 2
- Reconstituted Complex: 17
- Two-hybrid: 3
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 7
- Negative Genetic: 440
- Phenotypic Suppression: 4
- Positive Genetic: 70
- Synthetic Growth Defect: 6
- Synthetic Lethality: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SSC1/YJR045C is regulated by multiple transcription factors. AFT1, CST6, FKH2, GCR1, HSF1, MSN2, RAP1, SFP1, and SIN4 regulate SSC1 transcription under normal conditions. During the cellular response to heat, SSC1 transcription is regulated by FKH2, GCN5, HSF1 (positively), MED2, MED4, SPT3, SPT6, SUA7, TFC7, XBP1, and YAP1. Additionally, SFP1 regulates SSC1 transcription during the cellular response to stress.
- Regulators
- 18
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2009-08-06
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.