SIN3 / YOL004W Overview

Standard Name
SIN3 1
Systematic Name
CPE1 20 , GAM2 14 , RPD1 15 21 , SDI1 15 22 , SDS16 23 24 , UME4 15 25
Feature Type
ORF , Verified
Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity 2 3 4
Name Description
Switch INdependent
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
4999 +/- 2782
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all SIN3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Transcription cofactor subunit of the Rpd3L, Rpd3S, and Sin3-type histone deacetylase complexes (HDACs); regulates chromatin silencing, transcription by RNA polymerases I and II, DNA replication, meiosis, heat response, and the G2/M transition of the mitotic cell cycle; also involved in DNA repair

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutants grow slowly, have abnormal vacuolar morphology, decreased ethanol tolerance, short telomeres, short lifespan, large cell size, are impaired in respiratory growth and endocytosis, and are sensitive to caffeine, heat, various antibiotics; homozygous diploid nulls are defective in sporulation
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

2137 total interactions for 1186 unique genes

Physical Interactions

  • Affinity Capture-MS: 183
  • Affinity Capture-RNA: 10
  • Affinity Capture-Western: 17
  • Co-purification: 8
  • PCA: 1
  • Reconstituted Complex: 38
  • Two-hybrid: 20

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 1280
  • Phenotypic Enhancement: 27
  • Phenotypic Suppression: 22
  • Positive Genetic: 255
  • Synthetic Growth Defect: 138
  • Synthetic Lethality: 104
  • Synthetic Rescue: 33
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Sin3p regulates gene expression through its membership in the core Rpd3 class I histone deacetylase complex (HDAC) that contributes to chromatin stabilization and limits the accessibility of transcriptional factors to the underlying DNA. Sin3p is thought to provide structural support for the complex. The Rpd3 complex negatively regulates meiosis and is required for repression of certain meiotic genes during vegetative growth. Two distinct Rpd3 complexes have been identified: a small Rpd3S complex and a large Rpd3L complex. These two complexes share the three subunits, Rpd3p, Sin3p, and Ume1p in common.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-08-11

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.