HIR2 / YOR038C Overview
- Standard Name
- HIR2 1
- Systematic Name
- YOR038C
- SGD ID
- SGD:S000005564
- Aliases
- SPT1 11
- Feature Type
- ORF , Verified
- Description
- Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia 1 2 3 4 5 6 7 9
- Name Description
- HIstone Regulation 1 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 875
- Mol. Weight (Da)
- 98454.0
- Isoelectric Point
- 8.51
- Median Abundance (molecules/cell)
- 1417 +/- 495
- Half-life (hr)
- 8.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HIR2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcriptional corepressor that is part of the HIR complex; negatively regulates transcription of HTA1 and HTB1; localized to the nucleus and cytosol
View computational annotations
Molecular Function
- Manually Curated
- contributes to DNA binding (IDA)
- contributes to nucleosome binding (IMP)
- enables transcription corepressor activity (IDA)
Biological Process
- Manually Curated
- involved in chromatin organization (IDA)
- involved in negative regulation of transcription by RNA polymerase II (IDA)
- involved in negative regulation of transcription by RNA polymerase III (IGI, IMP)
- involved in transcription elongation-coupled chromatin remodeling (IGI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- acid pH resistance: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- competitive fitness: increased
- filamentous growth: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- invasive growth: absent
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- size of nucleolus: increased
- transposable element transposition: increased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
755 total interactions for 459 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 23
- Co-purification: 4
- Reconstituted Complex: 2
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 1
- Negative Genetic: 508
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 9
- Positive Genetic: 100
- Synthetic Growth Defect: 47
- Synthetic Lethality: 10
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Hir2p is a component of the HIR nucleosome assembly complex that regulates heterochromatic gene silencing and histone (HTA1, HTB1, HHT1, HHT2, HHF1, and HHF2) gene expression. The HIR nucleosome assembly complex is composed of four subunits, Hir1p, Hir2p, Hir3p and Hpc2p. HIR2 was first defined genetically as a transcriptional co-repressor of HTA1-HTB1 expression (with HIR1). Mutations in HIR1 and HIR2 act synergistically with mutations in Chromatin Assembly Factor I (CAF-1) to decrease telomeric and silencing at the HML loci. HIR associates with SWI/SNF complexes at histone gene promoters which are thought to relieve HIR-mediated repression and HIR complex binding to nucleosomes forms a distinct protein/DNA complex resistant to remodeling by SWI/SNF. The HIR1 and HIR2 genes are expressed constitutively under conditions that lead to repression or derepression of the HTA1 gene. Hir2p is observed in the nucleus but relocalizes to the cytosol in response to hypoxia.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-09-01
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.