New & Noteworthy

New Protein Half-life Data in SGD and YeastMine

September 08, 2016

Protein turnover for budding and fission yeast proteins, and scatterplot comparing homologous protein half-lives. Image from Cell Reports via Creative Commons license.

Ever wonder how quickly your favorite protein turns over within the cell? SGD has just incorporated half-life data for 3700 yeast proteins from a paper by Christiano et al., 2014. In this study, Christiano and colleagues pulse labeled exponentially growing wild type yeast cells in synthetic medium with a heavy lysine isotope (pulse SILAC), and followed the decay of native untagged proteins using high-resolution mass spectrometry based proteomics. The data generated in this study can be accessed by viewing the Experimental Data section of the Protein tab for your favorite gene, such as the short-lived Ctk1p or the long-lived Rsc1p.

In addition, you can retrieve this half-life data using YeastMine for one or more proteins with the Gene–>Protein Half-life template or obtain a list of proteins with half lives within a given range using the Retrieve–>Proteins with half-life in a given range template. Both of these templates can be found in the “Templates” section of YeastMine under the “Protein” category.

Thanks to Romaine Christiano and Tobias Walther for their help integrating this information into SGD.

Categories: New Data